Osawa K, Matsumoto T, Maruyama T, Naito Y, Okuda K, Takazoe I
Bull Tokyo Dent Coll. 1990 May;31(2):125-8.
Natural materials containing tannin were examined to determine whether they possessed any inhibitory activity against collagenase obtained from the culture supernatant of Bacteroides gingivalis. The collagenolytic activity was determined with Collagenokit CLN-100 (Collagen Technological Co., Tokyo) using FITC-conjugated collagen type 1. Among the test samples, only an aqueous extract of cacao (Theobroma cacao) bean husk strongly inhibited the bacterial collagenase. This inhibitory potency was at the same level as that of tetracycline-HCl.
对含有单宁的天然材料进行了检测，以确定它们是否对从牙龈拟杆菌培养上清液中获得的胶原酶具有任何抑制活性。使用FITC偶联的I型胶原，通过Collagenokit CLN-100（胶原蛋白技术公司，东京）测定胶原分解活性。在测试样品中，只有可可（Theobroma cacao）豆壳的水提取物强烈抑制细菌胶原酶。这种抑制效力与盐酸四环素相同。
