Osawa K, Matsumoto T, Yasuda H, Kato T, Naito Y, Okuda K
Department of Microbiology, Tokyo Dental College.
Bull Tokyo Dent Coll. 1991 Feb;32(1):1-7.
It is well known that plant extracts inhibit some enzymatic activities. The present study examined the inhibitory effects of natural plant extracts against the collagenolytic activity of Porphyromonas gingivalis. The enzyme was isolated from a culture supernatant of P. gingivalis 381. The aqueous and 50% ethanolic extracts of Ginkgo biloba, Mosla chinensis, Salvia officinalis, Cinnamomum cassia, and a catechin extract of Camellia sinensis exhibited strong inhibitory effects on collagenolytic activity. The activities of these plant extracts were higher than that of tetracycline-HCl. They also inhibited the cytotoxicity of P. gingivalis crude enzyme against human gingival fibroblasts. C. sinensis catechin was the most effective agent in neutralizing the cytotoxicity of P. gingivalis. The aqueous and 50% ethanolic extracts of C. cassia had relatively strong anti-cytotoxic activity. Although the other samples strongly inhibited the collagenolytic activity of P. gingivalis, they were not effectively anti-cytotoxic. The present findings suggest that C. sinensis and C. cassia extracts are effective in reducing the pathogenicity of periodontopathic bacteria.
众所周知，植物提取物能抑制某些酶的活性。本研究检测了天然植物提取物对牙龈卟啉单胞菌胶原酶活性的抑制作用。该酶是从牙龈卟啉单胞菌381的培养上清液中分离出来的。银杏、石香薷、鼠尾草、肉桂的水提取物和50%乙醇提取物以及茶树的儿茶素提取物对胶原酶活性均表现出强烈的抑制作用。这些植物提取物的活性高于盐酸四环素。它们还抑制牙龈卟啉单胞菌粗酶对人牙龈成纤维细胞的细胞毒性。儿茶素是中和牙龈卟啉单胞菌细胞毒性最有效的试剂。肉桂的水提取物和50%乙醇提取物具有较强的抗细胞毒性活性。虽然其他样品强烈抑制牙龈卟啉单胞菌的胶原酶活性，但它们没有有效的抗细胞毒性作用。目前的研究结果表明，茶树和肉桂提取物在降低牙周病原菌的致病性方面是有效的。
