Japan Health Sciences Foundation, Chuo-ku, Tokyo, Japan.
Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1236-1240. doi: 10.1099/ijs.0.016402-0. Epub 2009 Aug 7.
A strain of a novel pathogenic, achlorophyllic alga belonging to the genus Prototheca was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxonomic studies indicated that this strain represents a novel species with a close phylogenetic relationship to Prototheca wickerhamii and Auxenochlorella protothecoides. This strain grew well at 28-30 degrees C, showed slow and weak growth at 37 degrees C, and no growth at 40 degrees C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), l-arabinose and soluble starch as a carbon source. The taxonomic description of Prototheca cutis sp. nov. is proposed (type strain JCM 15793(T) =CBS 11262(T) =DSM 22084(T)) as a pathogen of dermatitis.
一株新型致病性、无叶绿素藻类菌株，属于 Prototheca 属，从日本一家医院患有 protothecosis 的患者发炎的皮肤中分离出来。通过组织病理学和基于培养的检查，在活检标本中检测和分离出病原体。核 18S rDNA 基因和 26S rDNA 基因 D1/D2 结构域序列分析和化学生态研究表明，该菌株代表一种新型物种，与 Prototheca wickerhamii 和 Auxenochlorella protothecoides 具有密切的系统发育关系。该菌株在 28-30°C 下生长良好，在 37°C 下生长缓慢且较弱，在 40°C 下不生长。该菌株在无维生素培养基中生长，并同化乙酸盐（pH5.1）、L-阿拉伯糖和可溶性淀粉作为碳源。提出了一种新型 Prototheca cutis sp. nov. 的分类描述（模式株 JCM 15793(T)=CBS 11262(T)=DSM 22084(T)），作为皮炎的病原体。
