Inoue J, Fukushima H, Onoe T, Eryu T, Ueda M, Yamaoka A, Sagawa H
Department of Bacteriology, Osaka Dental University.
Nihon Shishubyo Gakkai Kaishi. 1990 Mar;32(1):199-205. doi: 10.2329/perio.32.199.
Production of 9 enzymatic activities of 527 strains freshly isolated from periodontal pockets in advancing periodontitis were investigated. Of these isolates, two strains showed lecithinase activity on egg yolk agar plate. Collagenase, plasmin and lipase were produced by 28 strains, 26 strains and 22 strains, respectively. Two lecithinase-producing strains were identified as Bacteroides intermedius. Nineteen strains of B. intermedius and 1 strain of Fusobacterium species produced lipase on egg yolk agar plate. All of the 28 collagenase-producing strains were B. gingivalis. B. gingivalis (20 strains) and non black-pigmented Bacteroides (6 strains) showed plasmin activity. These results indicate that Bacteroides species, mainly B. gingivalis and B. intermedius may exert an important influence on the exacerbation of the disease.
对从进展期牙周炎牙周袋中新鲜分离出的527株菌株的9种酶活性进行了研究。在这些分离株中，有两株在蛋黄琼脂平板上显示出卵磷脂酶活性。分别有28株、26株和22株产生胶原酶、纤溶酶和脂肪酶。两株产生卵磷脂酶的菌株被鉴定为中间普氏菌。19株中间普氏菌和1株梭杆菌属菌株在蛋黄琼脂平板上产生脂肪酶。所有28株产生胶原酶的菌株均为牙龈卟啉单胞菌。牙龈卟啉单胞菌（20株）和非产黑色素普氏菌（6株）显示出纤溶酶活性。这些结果表明，普氏菌属，主要是牙龈卟啉单胞菌和中间普氏菌可能对疾病的加重发挥重要影响。
