[鸽胸肌蛋白激酶的分离及某些性质]
[Isolation and some properties of protein kinase from pigeon breast muscle].
作者信息
Iusupova G I, Grivennikov I A, Meshkova N P
出版信息
Biokhimiia. 1977 Jun;42(6):1062-8.
PMID:196686
Abstract
Protein kinase was isolated from pigeon breast muscle. The preparation obtained was chromatographically homogeneous. The apparent Km varlue for histone H1 and ATP were 3,5-10(-5) M and 1,6-10(-5) M respectively. The purified enzyme displays high specificity for the lysine-rich histones (H1, H2b, H2a). The protein kinase activity is stimulated, 1,6-fold by cyclic AMP.
摘要
蛋白激酶是从鸽胸肌中分离出来的。所得到的制剂在色谱上是均一的。组蛋白H1和ATP的表观Km值分别为3.5×10⁻⁵M和1.6×10⁻⁵M。纯化后的酶对富含赖氨酸的组蛋白（H1、H2b、H2a）表现出高度特异性。蛋白激酶活性被环磷酸腺苷刺激了1.6倍。
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