Basson J, Tardy J C, Aymard M
Laboratoire de Virologie, Centre Hospitalier Universitaire, Lyon, France.
J Clin Lab Immunol. 1990 Jan;31(1):43-9.
A C1q solid-phase enzyme assay for detecting circulating immune complexes (CIC) containing immunoglobulins M was developed. IgM immune complexes (IgM-CIC) bind to purified C1q-coated microplates. The amount of bound IgM-CIC is determined by adding anti-mu-alkaline phosphatase conjugate. This assay proved IgM-CIC specific; it does not detect IgG-CIC nor free anti-CMV IgM. Furthermore Rheumatoid Factor (RF) does not interfere with this test. Then, 119 renal allograft recipients were followed-up for the presence of seric IgM-CIC. Among them, 86 patients developed a recurrent Cytomegalovirus (CMV) infection and 33 did not. In the non-infected population, IgM-CIC were detected in only 15.2% of the patients. In contrast, patients with recurrent CMV infection showed a significantly higher frequency of detectable IgM-CIC (62.8%) (p less than 0.01). These IgM-CIC were detected: (a) during the first two or three weeks after graft; (b) in the course of the second month post graft. This second peak of IgM-CIC was never observed in subjects non-infected with CMV. IgM-CIC occurred before or at the same time as the detectable anti-CMV IgM and virus excretion in urines. Presence of IgM-CIC was not influenced by graft rejection episodes. Such a marker might help in discriminating immune response to viral infection from immune graft rejection.
我们开发了一种用于检测含有免疫球蛋白M的循环免疫复合物（CIC）的C1q固相酶测定法。IgM免疫复合物（IgM-CIC）与纯化的C1q包被微孔板结合。通过加入抗μ-碱性磷酸酶结合物来测定结合的IgM-CIC的量。该测定法证明对IgM-CIC具有特异性；它不检测IgG-CIC，也不检测游离的抗巨细胞病毒（CMV）IgM。此外，类风湿因子（RF）不干扰该检测。然后，对119例肾移植受者进行随访，以检测血清中IgM-CIC的存在情况。其中，86例患者发生了复发性巨细胞病毒（CMV）感染，33例未发生。在未感染人群中，仅15.2%的患者检测到IgM-CIC。相比之下，复发性CMV感染患者中可检测到的IgM-CIC频率显著更高（62.8%）（p小于0.01）。这些IgM-CIC在以下情况被检测到：（a）移植后的头两到三周内；（b）移植后第二个月期间。在未感染CMV的受试者中从未观察到IgM-CIC的第二个峰值。IgM-CIC在可检测到的抗CMV IgM和尿液中病毒排泄之前或同时出现。IgM-CIC的存在不受移植排斥反应发作的影响。这样一种标志物可能有助于区分对病毒感染的免疫反应和免疫移植排斥反应。
