Sokolenko A A, Shakhanina K L, Kurilov A N
Biull Eksp Biol Med. 1992 Feb;113(2):174-6.
A complex enzyme immunoassay (ELISA) has been designed for antigen-specific determination of HBsAg-containing circulating immune complexes (CIC HBsAg/IgM and CIC HBsAg/IgG) in human blood sera in parallel with registration of free HBsAg and specific antibodies to viruses of hepatitis A, B and D. It is shown that effective formation of HBsAg-containing CIC serologically is registered predominantly as a mutually incompatible marker with detection of free HBsAg (in 70-85% of the cases). CIC HBsAg/IgM and CIC HBsAg/IgG may be registered both in parallel and as mutually exclusive markers. Effective formation of HBsAg-containing CIC in the presence of anti-HBsAg occurs in case of a mild course of viral hepatitis of epidemic and sporadic type, while in severe forms of VH-free HBsAg is predominantly detected thus pointing either to ineffective formation of HBsAg-containing CIC or to their continuous registration with demonstration of the effect of delay of witching of anti-HBsM over to anti-HBsG (or CIC HBsAg/IgM to CIC HBsAg/IgG). It was also found that in case of epidemic VH in Tajik SSR (1987) serologically marked as VH both A and B convalescent phase was characterized by parallel disappearance (or lowering of the titer levels) of HBsAg-containing CIC and class M antibodies to both hepatitis A (anti-HAV M) and B (anti-HBcM, anti-HBsM) along with the containing parallel registration of relevant G-antibodies (anti-HAV G/anti-HBcG). This observation requires further studies both in terms of close association of viruses of hepatitides A and B and with regards to possible antigenic mimicry.
已设计出一种复杂的酶免疫测定法（ELISA），用于在人血清中抗原特异性测定含乙肝表面抗原（HBsAg）的循环免疫复合物（CIC HBsAg/IgM和CIC HBsAg/IgG），同时记录游离HBsAg以及甲型、乙型和丁型肝炎病毒的特异性抗体。结果表明，含HBsAg的CIC的有效血清学形成主要作为与游离HBsAg检测相互不兼容的标志物被记录（在70 - 85%的病例中）。CIC HBsAg/IgM和CIC HBsAg/IgG既可以并行记录，也可以作为相互排斥的标志物记录。在流行性和散发性病毒性肝炎病程较轻的情况下，在存在抗HBsAg时会发生含HBsAg的CIC的有效形成，而在严重形式的病毒性肝炎中，主要检测到无游离HBsAg，这表明要么含HBsAg的CIC形成无效，要么它们持续被记录，显示出抗HBsM向抗HBsG转换延迟的效应（或CIC HBsAg/IgM向CIC HBsAg/IgG转换延迟的效应）。还发现，在塔吉克苏维埃社会主义共和国1987年的流行性病毒性肝炎病例中，血清学上标记为甲型和乙型肝炎恢复期的阶段，其特征是含HBsAg的CIC以及甲型肝炎（抗HAV M）和乙型肝炎（抗HBcM、抗HBsM）的M类抗体并行消失（或滴度水平降低），同时相关G类抗体（抗HAV G/抗HBcG）并行记录。这一观察结果在甲型和乙型肝炎病毒的密切关联以及可能的抗原模拟方面都需要进一步研究。
