Steinitz M, Ingvarsson S, Asker C, Sümegi J, Klein G
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
J Clin Lab Immunol. 1990 Jun;32(2):49-54.
An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.
构建了一个带有Bmyc癌基因完整编码区的表达载体。Bmyc最长的预测开放阅读框（178个氨基酸残基）在转化细菌中与trpE蛋白（308个氨基酸残基）融合表达为一种融合蛋白。新合成的58 Kd蛋白与抗泛myc血清发生反应。从制备性蛋白质免疫印迹中分离出的融合蛋白用作免疫原，以产生兔抗myc特异性免疫血清。
