Asker C, Steinitz M, Andersson K, Sümegi J, Klein G, Ingvarsson S
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Oncogene. 1989 Dec;4(12):1523-7.
We have cloned and sequenced the rat Bmyc gene. The rat Bmyc gene contains sequences related to the central part of c-myc, namely the first intron, the second exon, and the noncoding part of the third exon. The homology drops in the 3' part of the c-myc second exon, but continues in the noncoding part of the third exon. We have sequenced the total predicted coding region of the Bmyc. The longest open reading frame in Bmyc suggests a protein of 178 amino acids, which is only 41% of the c-myc protein size. To confirm the putative open reading frame, we have produced a trpE-Bmyc protein that is detected with a pan-myc antibody. We discuss these findings in the context of potential functional domains and the possibility of overlapping and distinct activities of myc-family proteins.
我们已经克隆并测序了大鼠Bmyc基因。大鼠Bmyc基因包含与c-myc中央部分相关的序列，即第一个内含子、第二个外显子以及第三个外显子的非编码部分。在c-myc第二个外显子的3'部分同源性下降，但在第三个外显子的非编码部分仍继续存在。我们已经对Bmyc的全部预测编码区进行了测序。Bmyc中最长的开放阅读框提示有一个由178个氨基酸组成的蛋白质，其大小仅为c-myc蛋白质大小的41%。为了证实推定的开放阅读框，我们制备了一种用泛myc抗体可检测到的trpE-Bmyc蛋白。我们在潜在功能域以及myc家族蛋白重叠和不同活性可能性的背景下讨论了这些发现。
