玉米激活子元件在烟草中优先转座至连锁的染色体位点。
Preferential transposition of the maize element Activator to linked chromosomal locations in tobacco.
作者信息
Jones J D, Carland F, Lim E, Ralston E, Dooner H K
机构信息
DNA Plant Technology, Oakland, California 94608.
出版信息
Plant Cell. 1990 Aug;2(8):701-7. doi: 10.1105/tpc.2.8.701.
Abstract
The autonomous maize transposon Activator (Ac) has been used in maize for gene isolation by tagging and may prove similarly useful in other species. To test the feasibility of gene tagging with heterospecific transposons, we have examined three key genetic properties of a slightly modified Ac in tobacco. First, we show that frequencies of germinal excision of this Ac element from the antibiotic resistance gene streptomycin phosphotransferase can be comparable with or slightly lower than in maize. Second, we show that about half of the progeny carrying a germinal excision product also carry a transposed Ac. Last, we have mapped transposed Ac locations relative to the streptomycin transferase gene excision product and have shown that as in maize Ac in tobacco preferentially transposes to genetically linked sites.
摘要
自主玉米转座子激活子（Ac）已在玉米中用于通过标签法分离基因，并且可能在其他物种中同样有用。为了测试用异源转座子进行基因标签的可行性，我们研究了烟草中一种经过轻微修饰的Ac的三个关键遗传特性。首先，我们表明这种Ac元件从抗生素抗性基因链霉素磷酸转移酶上的生殖切除频率与玉米中的相当或略低。其次，我们表明携带生殖切除产物的后代中约有一半也携带转座的Ac。最后，我们绘制了转座的Ac相对于链霉素转移酶基因切除产物的位置，并表明与玉米中一样，烟草中的Ac优先转座到遗传连锁位点。
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本文引用的文献
1
Visual detection of transposition of the maize element activator (ac) in tobacco seedlings.玉米转座子激活因子（ac）在烟草幼苗中的可视化检测。
Science. 1989 Apr 14;244(4901):204-7. doi: 10.1126/science.244.4901.204.
2
Mutable R-navajo alleles of cyclic origin in maize.玉米中环型起源的可变性 R-navajo 等位基因。
Genetics. 1973 Feb;73(2):273-96. doi: 10.1093/genetics/73.2.273.
3
The Relation between Light Variegated and Medium Variegated Pericarp in Maize.玉米浅花斑与中花斑果皮之间的关系
Genetics. 1952 Sep;37(5):519-44. doi: 10.1093/genetics/37.5.519.
4
Transposition Pattern of the Maize Element Ac from the Bz-M2(ac) Allele.玉米 Bz-M2(ac) 等位基因 Ac 元件的转座模式。
Genetics. 1989 Jun;122(2):447-57. doi: 10.1093/genetics/122.2.447.
5
Genetic Fine Structure of the BRONZE Locus in Maize.玉米 BRONZE 基因座的遗传精细结构。
Genetics. 1986 Aug;113(4):1021-36. doi: 10.1093/genetics/113.4.1021.
6
Transposition of the maize controlling element "Activator" in tobacco.玉米调控元件“激活子”在烟草中的转位。
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844-8. doi: 10.1073/pnas.83.13.4844.
7
Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac).利用转座调控元件激活因子（Ac）通过简单且可推广的程序克隆玉米中的青铜基因座。
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3825-9. doi: 10.1073/pnas.81.12.3825.
8
Characterization of the maize transposable element Ac by internal deletions.通过内部缺失对玉米转座子 Ac 进行特征分析。
EMBO J. 1988 Dec 1;7(12):3653-9. doi: 10.1002/j.1460-2075.1988.tb03246.x.
9
Transcription of transposable element Activator (Ac) of Zea mays L.玉米转座因子 Activator（Ac）的转录
EMBO J. 1987 Jun;6(6):1555-63. doi: 10.1002/j.1460-2075.1987.tb02400.x.
10
Phenotypic assay for excision of the maize controlling element Ac in tobacco.玉米控制元件 Ac 在烟草中的切除表型分析。
EMBO J. 1987 Jun;6(6):1547-54. doi: 10.1002/j.1460-2075.1987.tb02399.x.
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