Suga M, Ando M, Tanaka F, Araki S
Kumamoto Rosai Hospital, Japan.
J Clin Lab Immunol. 1990 Oct;33(2):55-9.
We studied the triggering effects of opsonized-IgG antibody on the superoxide (O2-) release and phagosome-lysosome fusion in the phagosome of pulmonary alveolar macrophages (PAM). Both were histochemically assayed in the same cell to enable simultaneous observation, using the qualitative superoxide dismutase (SOD)-inhibitable nitroblue tetrazolium (NBT) reduction test and the acridine orange (AO) method. When PAM from normal rabbit lungs were made to phagocytize the IgG-treated yeasts, the cells showed the enhanced release of O2- which acted directly on the antibody-coated yeasts as an oxidant, and the enhanced phagosome-lysosome fusion that was inhibited by SOD and was closely related to the enhanced release of O2- in the phagosomes. In contrast, neither enhanced O2- release nor phagosome-lysosome fusion was observed in the PAM which phagocytized saline-treated yeasts. These results may suggest that the opsonized-IgG antibody enhances the O2- release in the phagosome, resulting in the enhanced phagosome-lysosome fusion.
我们研究了调理素化IgG抗体对肺泡巨噬细胞（PAM）吞噬体中超氧化物（O2-）释放和吞噬体-溶酶体融合的触发作用。使用定性超氧化物歧化酶（SOD）抑制的氮蓝四唑（NBT）还原试验和吖啶橙（AO）方法，在同一细胞中对两者进行组织化学分析，以便能够同时观察。当来自正常兔肺的PAM吞噬经IgG处理的酵母时，细胞显示出O2-释放增强，其作为氧化剂直接作用于抗体包被的酵母，并且吞噬体-溶酶体融合增强，该融合被SOD抑制且与吞噬体中O2-释放增强密切相关。相反，在吞噬经盐水处理的酵母的PAM中，未观察到O2-释放增强或吞噬体-溶酶体融合增强。这些结果可能表明，调理素化IgG抗体增强了吞噬体中的O2-释放，从而导致吞噬体-溶酶体融合增强。
