Vandenbroucke-Grauls C M, Thijssen R M, Marcelis J H, Sharma S D, Verhoef J
Immunology. 1984 Feb;51(2):319-26.
Lysosomotropic agents interfere with lysosome function. We studied the effects of the lysosomotropic amines: lidocaine, diphenylamine and dansylcadaverine on several functions of human polymorphonuclear leucocytes (PMN): enzyme release, phagosome-lysosome fusion, superoxide anion generation upon stimulation with opsonized bacteria, and phagocytosis and killing of opsonized Staphylococcus aureus. Lidocaine depressed all cellular functions tested. Diphenylamine reduced enzyme release and phagosome-lysosome fusion in phagocytosing PMN. This was accompanied by an increase in superoxide anion generation. Dansylcadaverine enhanced enzyme release and phagosome-lysosome fusion, and reduced superoxide anion generation. Neither of these two agents influenced bacterial uptake; bacterial killing was impaired only in dansylcadaverine treated cells. Cadaverine, an analogue that does not penetrate cells, had no effect on any of the functions tested.
溶酶体亲和剂会干扰溶酶体功能。我们研究了溶酶体亲和胺类物质：利多卡因、二苯胺和丹磺酰尸胺对人多形核白细胞（PMN）几种功能的影响：酶释放、吞噬体 - 溶酶体融合、经调理细菌刺激后的超氧阴离子生成，以及对调理过的金黄色葡萄球菌的吞噬和杀伤作用。利多卡因抑制了所有测试的细胞功能。二苯胺减少了吞噬PMN中的酶释放和吞噬体 - 溶酶体融合。这伴随着超氧阴离子生成的增加。丹磺酰尸胺增强了酶释放和吞噬体 - 溶酶体融合，并减少了超氧阴离子生成。这两种物质都不影响细菌摄取；只有在经丹磺酰尸胺处理的细胞中细菌杀伤受到损害。尸胺，一种不能穿透细胞的类似物，对任何测试功能均无影响。
