Lu Dezhao, Wo Xingde, Wo Like, Li Yi, Tang Lihua, Yang Zhen
College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2009 May;34(10):1251-6.
To study the effects of warm and tonify kidney-yang herbs on the liver mitochondria proteome of the thyroidectomized kidney-yang deficiency rats.
Twelve rats were divided into normal group, model group and treated group; each group had four rats. The rats of model group and treated group were excised the two side thyroid gland, and the rats of normal group were done the homologous operation, but didn't excise the thyroid gland. After seven days, the rats of model group and treated group appeared the symptoms of kidney-yang deficiency. From the eighth day, the rats of treated group were fed warm and tonify kidney-yang herbs 6.7 g x kg(-1) once daily, and the rats of other two groups were fed the equal normal. All rats of three groups were killed by decollation after six days of treatment, and liver mitochondria proteins were separated. Each liver mitochondria protein sample was labeled with Cy3 or Cy5 randomly, and one Cy3-labeled sample and one Cy5-labeled sample were mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard and run on the same gel. The gels were scanned under different wavelength light after electrophoresis. All images were analyzed by DeCyder 6. 5 software, and the different proteins were identified by mass spectrum.
The expression of HSP60, catalase, glutathione peroxidase, carbamoylphosphate synthetase I, ATP synthase, lactotransferrin, H(+)-transporting two-sector ATPase, alpha-ETF and calpain 12 were increased in the thyroidectomized kidney-yang deficiency rats, while the expression of oxoisovalerate dehydrogenase, Acyl-CoA dehydrogenase, ornithine aminotransferase, and GTP-binding regulatory protein were decreased. After the kidney-yang deficiency rats were treated with warm and tonify kidney-yang herbs, the expression of HSP60, catalase, glutathione peroxidase, oxoisovalerate dehydrogenase, Acyl-CoA dehydrogenase, ATP synthase, lacto-transferrin, H(+)-transporting two-sector ATPase, alpha-ETF and GTP-binding regulatory protein were increased, and the expression of carbamoyl-phosphate synthetase I and calpain 12 were decreased.
The warm and tonify kidney-yang herbs perform its therapeutical effect by regulating the metabolism, protecting the stability of mitochondrial membrance and maintaining the signal conduction in the cells.
研究温补肾阳中药对甲状腺切除肾阳虚大鼠肝脏线粒体蛋白质组的影响。
将12只大鼠分为正常组、模型组和治疗组，每组4只。模型组和治疗组大鼠切除双侧甲状腺，正常组大鼠行同样手术但不切除甲状腺。7天后，模型组和治疗组大鼠出现肾阳虚症状。从第8天起，治疗组大鼠每日灌胃温补肾阳中药6.7 g·kg⁻¹，其他两组大鼠灌胃等量生理盐水。治疗6天后，三组大鼠均断头处死，分离肝脏线粒体蛋白质。每个肝脏线粒体蛋白质样品随机用Cy3或Cy5标记，将一个Cy3标记样品和一个Cy5标记样品与所有样品的Cy2标记混合物作为内标混合在同一块二维凝胶上，并在同一块凝胶上进行电泳。电泳后在不同波长光下扫描凝胶。所有图像用DeCyder 6.5软件分析，不同蛋白质用质谱鉴定。
甲状腺切除肾阳虚大鼠中，热休克蛋白60（HSP60）、过氧化氢酶、谷胱甘肽过氧化物酶、氨甲酰磷酸合成酶I、ATP合酶、乳铁传递蛋白、H⁺转运双功能ATP酶、α-电子传递黄素蛋白（alpha-ETF）和钙蛋白酶12的表达增加，而氧代异戊酸脱氢酶、酰基辅酶A脱氢酶、鸟氨酸氨基转移酶和GTP结合调节蛋白的表达降低。肾阳虚大鼠用温补肾阳中药治疗后，HSP60、过氧化氢酶、谷胱甘肽过氧化物酶、氧代异戊酸脱氢酶、酰基辅酶A脱氢酶、ATP合酶、乳铁传递蛋白、H⁺转运双功能ATP酶、alpha-ETF和GTP结合调节蛋白的表达增加，氨甲酰磷酸合成酶I和钙蛋白酶12的表达降低。
温补肾阳中药通过调节代谢、保护线粒体膜稳定性和维持细胞内信号传导发挥治疗作用。
