Li Xiangping, Yin Tao, Zhong Guangwei, Li Wei, Luo Yanhong, Xiang Lingli, Liu Zhehao
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1997-2004.
To observe the herbal effects on hyperthyroidism patients with syndrome of hyperactivity of liver-Yang by method for calming the liver and suppressing Yang and investigate its effects on the lymphocyte protein expression. This approach may lay a foundation for the further investigation of the curative mechanisms of calming the liver and suppressing Yang treatment.
A total of 48 hyperthyroidism patients with syndrome of hyperactivity of liver-Yang were randomly divided into treatment group and control group. The treatment group was treated by method for calming the liver and suppressing Yang in accordance with traditional Chinese medicine (TCM) and the control group with thiamazole tablets for three periods of treatment The therapeutic effects, the score of TCM symptom, electrocardiogram (P wave), thyroid hormones and ultrasound were observed in both groups before and after the treatment. The side effects in the treatment course were observed in both groups. The level of differential protein expression was analyzed by two-dimensional electrphoresis and matrix assisted laser desorption/ionizaton time-of-flight mass spectrometry.
The treatment group has the effect on stepping down the heart rate, cutting down the P wave amplitude changes, regulating the level of thyroid hormones and decreasing the volume of thyromegaly. There are not statistically significant between the treatment group and control group. However, the treatment group has obviously better effect on regulating TCM symptom and decreasing the side reaction than the control group (P<0.05). There are not statistically significant on the total effective between the treatment group and control group. The average spots in lymphocyte for normal people, before and after treating hyperthyroidism patients with syndrome of hyperactivity of liver-Yang were (429 +/- 31), (452 +/- 28) and (437 +/- 36) spots respectively. Eight down-regulated protein expressions and 11 up-regulated protein expressions were obtained in the hyperthyroidism patients with syndrome of hyperactivity of liver-Yang and normal people. Five strengthened expressions of protein were also obtained in 8 down-regulated expressions of protein and 8 lower expressions of protein in 11 up-regulated expressions of protein before and after treating the migraine patients with syndrome of hyperactivity of liver-Yang. Ten of the total 8 differential protein spots were successfully identified by MALDI-TOF-MS. The functions of these proteins were involved in metabolism associated, transportation, antioxidation, sigal transduction and immume associated protein, etc. according to information provided by NCBI and MSDB database.
In this study, the TCM complex prescription with herbs for calming the liver and suppressing Yang can regulate the thyroid hormones, improves TCM symptoms, and decrease the adverse reaction. It can possibly regulate lymphocyte protein expression.
观察平肝潜阳法对甲状腺功能亢进症肝阳上亢证患者的作用，并探讨其对淋巴细胞蛋白表达的影响，为进一步研究平肝潜阳法的治疗机制奠定基础。
将48例甲状腺功能亢进症肝阳上亢证患者随机分为治疗组和对照组。治疗组采用平肝潜阳法进行中医治疗，对照组采用甲巯咪唑片治疗，疗程均为3个阶段。观察两组治疗前后的疗效、中医症状评分、心电图（P波）、甲状腺激素及甲状腺超声情况，并观察两组治疗过程中的不良反应。采用二维电泳和基质辅助激光解吸/电离飞行时间质谱分析差异蛋白表达水平。
治疗组在降低心率、减小P波振幅变化、调节甲状腺激素水平及缩小甲状腺肿大体积方面有一定作用，与对照组比较差异无统计学意义。但治疗组在调节中医症状及减少不良反应方面明显优于对照组（P<0.05）。两组总有效率比较差异无统计学意义。正常人、甲状腺功能亢进症肝阳上亢证患者治疗前后淋巴细胞平均蛋白点数分别为（429±31）、（452±28）、（437±36）个。甲状腺功能亢进症肝阳上亢证患者与正常人相比，有8个蛋白表达下调，11个蛋白表达上调。甲状腺功能亢进症肝阳上亢证患者治疗前后，8个下调蛋白表达中有5个蛋白表达增强，11个上调蛋白表达中有8个蛋白表达降低。经基质辅助激光解吸/电离飞行时间质谱成功鉴定出8个差异蛋白点中的10个。根据美国国立生物技术信息中心和质谱数据库提供的信息，这些蛋白的功能涉及代谢相关、转运、抗氧化、信号转导及免疫相关蛋白等。
本研究中平肝潜阳复方中药能调节甲状腺激素，改善中医症状，减少不良反应，可能对淋巴细胞蛋白表达有调节作用。
