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通过高效液相色谱-电喷雾电离质谱法测定糖化血红蛋白A1c的国际临床化学和检验医学联合会参考测量程序的修改

Modification of the IFCC reference measurement procedure for determination of HbA 1c by HPLC-ESI-MS.

作者信息

Kaiser Patricia, Akerboom Theodorus, Dux László, Reinauer Hans

机构信息

Reference Measurement Laboratory, Instand e.V., Düsseldorf, Germany.

出版信息

Ger Med Sci. 2006 Sep 21;4:Doc06.

PMID:19675697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2703220/
Abstract

The reference measurement procedure for determination of HbA(1c) (glycated haemoglobin) using HPLC(high performance liquid chromatography)-ESI(electrospray ionisation)-MS(mass spectrometry) has been modified. Main modifications were a change in the buffer composition of the HPLC, a change in the gradient elution profile and the introduction of a post-column splitting system. The long-term stability of the HPLC-ESI-MS system proved to be of high importance to get reproducible results. Using the modified HPLC conditions stable retention times, high peak symmetry and a good column batch-to-batch reproducibility of the analytical results were obtained. The mean deviation from the target values was less than 0.7% with an imprecision of less than 2%.

摘要

采用高效液相色谱（HPLC）-电喷雾电离（ESI）-质谱（MS）法测定糖化血红蛋白（HbA(1c)）的参考测量程序已得到改进。主要改进包括HPLC缓冲液成分的改变、梯度洗脱曲线的变化以及柱后分流系统的引入。事实证明，HPLC-ESI-MS系统的长期稳定性对于获得可重复的结果至关重要。采用改进后的HPLC条件，可获得稳定的保留时间、较高的峰对称性以及各批次色谱柱之间良好的分析结果重现性。与目标值的平均偏差小于0.7%，不精密度小于2%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/c8cdcafa1a73/GMS-04-06-g-005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/c457836c7e9b/GMS-04-06-t-002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/5c601a2ce1bf/GMS-04-06-t-003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/888bb112de7f/GMS-04-06-t-004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/5f7345308b0b/GMS-04-06-t-005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/2bd89a7f7c82/GMS-04-06-t-006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/c73cec998833/GMS-04-06-g-001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/f7a0f5b8da95/GMS-04-06-g-002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/003cbe72308d/GMS-04-06-g-003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/92a620f88a95/GMS-04-06-g-004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23bc/2703220/c8cdcafa1a73/GMS-04-06-g-005.jpg

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Preparation of a candidate primary reference material for the international standardisation of HbA1c determinations.糖化血红蛋白（HbA1c）测定国际标准化候选一级参考物质的制备
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J Clin Lab Anal. 2016 Sep;30(5):457-63. doi: 10.1002/jcla.21879. Epub 2015 Oct 29.
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Diabetes mellitus: the long way of standardization of HbA(1c) to the level of highest metrological order.糖尿病：将糖化血红蛋白（HbA₁c）标准化至最高计量级水平的漫长之路。
Ger Med Sci. 2011;9:Doc28. doi: 10.3205/000151. Epub 2011 Nov 9.