Ismail Rasha S A, El-Megeid Ashraf A A, Abdel-Moemin Aly R
Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt.
Ger Med Sci. 2009 Jun 30;7:Doc05. doi: 10.3205/000064.
The aim of the present investigation was to study the effects of olive oil (OO), corn oil (CO), and flaxseed oil (FO), with or without supplementation of vitamins E and C, on food intake, body weight gain %, liver weight to body weight %, total lipids, liver functions, and liver histology in male rats intoxicated with carbon tetrachloride (CCl(4)). Forty-two rats were divided into two main groups. The first main group was fed on basal diet (BD) as a negative control group (NC). The second main group received subcutaneous injections of CCl(4) in paraffin oil (50% v/v 2 ml/kg) twice a week to induce chronic damage in the liver. The group was then divided into six subgroups, three of which were fed on 4% unsupplemented oils (CO, FO, and OO) as positive control for the three oils used. The rest of the groups were fed on 4% of the same oils supplemented with vitamins E and C. The results of the flaxseed oil rat group indicate that supplementing vitamin E and C led to a significant reduction in the mean values of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), and liver alanine amino transferase enzyme (ALT). Moreover, it caused an increase of the mean value of high-density lipoprotein cholesterol (HDL-C) as compared to the negative control group (NC). The olive oil group supplemented with the same vitamins showed a significant decrease in the mean value of serum TC and significant (P<0.05) increase in the mean value of serum HDL-C as compared to NC. The results of the corn oil group supplemented with vitamins showed a significant increase in the mean value of serum HDL-C as compared to the negative control group. The histology results confirmed that the group hepatically injured with CCl(4) treatment and fed on supplemented FO or OO showed apparently normal hepatocytes.
The most effective treatment was observed with oils supplemented with vitamins E and C. Hierarchically FO achieved the best results compared to other additives, followed by OO and finally CO showing the least effective treatment among the observed groups.
本研究的目的是研究橄榄油（OO）、玉米油（CO）和亚麻籽油（FO），无论是否添加维生素E和C，对四氯化碳（CCl₄）中毒雄性大鼠的食物摄入量、体重增加百分比、肝重与体重百分比、总脂质、肝功能和肝脏组织学的影响。42只大鼠分为两个主要组。第一个主要组喂食基础饮食（BD）作为阴性对照组（NC）。第二个主要组每周两次皮下注射溶于石蜡油的CCl₄（50% v/v 2 ml/kg）以诱导肝脏慢性损伤。然后该组再分为六个亚组，其中三个亚组喂食4%未添加维生素的油（CO、FO和OO）作为所使用的三种油的阳性对照组。其余组喂食添加了维生素E和C的4%相同的油。亚麻籽油大鼠组的结果表明，补充维生素E和C导致总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和肝丙氨酸转氨酶（ALT）的平均值显著降低。此外，与阴性对照组（NC）相比，它导致高密度脂蛋白胆固醇（HDL-C）的平均值增加。补充相同维生素的橄榄油组与NC相比，血清TC平均值显著降低，血清HDL-C平均值显著（P<0.05）增加。补充维生素的玉米油组与阴性对照组相比，血清HDL-C平均值显著增加。组织学结果证实，用CCl₄处理并喂食补充了FO或OO的肝脏损伤组显示肝细胞明显正常。
观察到补充维生素E和C的油治疗效果最佳。分层来看，与其他添加剂相比，FO取得了最佳效果，其次是OO，最后是CO，在所观察的组中显示出最无效的治疗效果。
