Al-Attar Atef M, Shawush Nessreen A
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 139109, Jeddah 21323, Saudi Arabia.
Saudi J Biol Sci. 2014 Nov;21(5):473-80. doi: 10.1016/j.sjbs.2014.08.004. Epub 2014 Sep 6.
Physiologically, it is known that thioacetamide (TAA) toxicity is generally associated with hepatic fibrosis induction, complicated metabolic disorders and health problems. The capability of extracts of olive and rosemary leaves to attenuate the severe physiological disturbances induced by thioacetamide (TAA) intoxication in male rats has been evaluated. Healthy male Wistar rats were used in the present study and were divided randomly into eight groups. Rats of the first group were served as normal control. Rats of the second group were administrated with TAA. Rats of the third, fourth and fifth groups were exposed to TAA plus olive leaves extract, TAA plus rosemary leaves extract and TAA plus olive and rosemary leaves extracts respectively. The sixth, seventh and eighth groups were supplemented with olive leaves extract, rosemary leaves extract, and olive and rosemary leaves extracts respectively. After 12 weeks of experimental treatments, the levels of serum glucose, total protein, albumin and high density lipoprotein cholesterol were significantly decreased, while the levels of triglycerides, cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, creatine kinase and lactate dehydrogenase were statistically increased in rats exposed to TAA. Administration of the studied extracts inhibited the hematobiochemical parameters and improved the physiological disturbances induced by TAA intoxication. Additionally, most improvements were noted in rats administrated with rosemary leaves extract followed by olive and rosemary leaves extracts and olive leaves extract. These results suggested that the effect of these extracts might be due to their antioxidant activities against TAA toxicity.
从生理角度来看，已知硫代乙酰胺（TAA）毒性通常与肝纤维化诱导、复杂的代谢紊乱及健康问题相关。已评估了橄榄叶和迷迭香叶提取物减轻硫代乙酰胺（TAA）中毒对雄性大鼠造成的严重生理紊乱的能力。本研究使用健康雄性Wistar大鼠，并将其随机分为八组。第一组大鼠作为正常对照。第二组大鼠给予TAA。第三、第四和第五组大鼠分别暴露于TAA加橄榄叶提取物、TAA加迷迭香叶提取物以及TAA加橄榄叶和迷迭香叶提取物。第六、第七和第八组大鼠分别补充橄榄叶提取物、迷迭香叶提取物以及橄榄叶和迷迭香叶提取物。经过12周的实验处理后，暴露于TAA的大鼠血清葡萄糖、总蛋白、白蛋白和高密度脂蛋白胆固醇水平显著降低，而甘油三酯、胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇、肌酸激酶和乳酸脱氢酶水平在统计学上有所升高。给予所研究的提取物可抑制血液生化参数，并改善TAA中毒引起的生理紊乱。此外，在给予迷迭香叶提取物的大鼠中观察到的改善最为明显，其次是给予橄榄叶和迷迭香叶提取物以及橄榄叶提取物的大鼠。这些结果表明，这些提取物的作用可能归因于它们对TAA毒性的抗氧化活性。
