Lopes K A R, Campos Velho N M R, Munin E
Centro de Estudos da Natureza, Universidade do Vale do Paraíba, São José dos Campos, SP, Brazil, 12244-000.
Braz J Biol. 2009 May;69(2):327-32. doi: 10.1590/s1519-69842009000200013.
The mechanism of regeneration does not start to restore the wound until its corresponding epimorphic phase. A bioestimulation of tissues and cells by laser radiation depends on the wavelength, on the dose, and on the intensity of the light. The goal of this work was to verify the effect of the low power laser at 660 nm on the regenerative process of Girardia tigrina. The specimens were maintained in the laboratory under a temperature ranging from 19 degrees up to 24 degrees C for 21 days. The planarians were anesthetized by placing them on ice and then cut them with a scalpel. The three treatments were as following: animals individually irradiated with 14 sessions with 1 minute duration (treatment 1), 14 sessions with 3 minutes duration (treatment 2), and without irradiation (control). The planarians were amputated and divided in three study treatments: a control group (without radiation), and two other treatments: irradiated for 1 minute, and irradiated for 3 minutes. The animals were irradiated with diode laser (660 nm) with 3.3 +/- 0.3 mW of power, using 0.94 mW.mm-2 power density for each irradiation procedure. During the experiment, 14 irradiation sessions were undertaken. The specimens were fixed in Bouin, and stained with hematoxyline and eosin. From observation and histological analysis, it was possible to assess the effects of interaction between laser and tissue. The head fragment after 1 minute of irradiation presented a better organized tissue scheme, when compared with the other treatments. Aspects of the body fragments submitted to 3 minutes of light treatment were very similar to fragments that had not been injured. It can be concluded that there are changes in the quality of regeneration when treated with low power laser under the conditions mentioned above.
再生机制在其相应的形态发生阶段之前不会开始修复伤口。激光辐射对组织和细胞的生物刺激取决于波长、剂量和光强度。这项工作的目的是验证660nm低功率激光对虎纹涡虫再生过程的影响。标本在实验室中于19摄氏度至24摄氏度的温度下保存21天。将涡虫放在冰上麻醉，然后用手术刀切割。三种处理如下：动物分别接受14次每次1分钟的照射（处理1）、14次每次3分钟的照射（处理2）以及不进行照射（对照）。将涡虫截肢并分为三种研究处理：一个对照组（不进行辐射），以及另外两种处理：照射1分钟和照射3分钟。使用功率为3.3±0.3mW的二极管激光（660nm）对动物进行照射，每次照射程序的功率密度为0.94mW·mm-2。在实验过程中，进行了14次照射。标本用Bouin固定液固定，并用苏木精和伊红染色。通过观察和组织学分析，可以评估激光与组织之间相互作用的效果。与其他处理相比，照射1分钟后的头部片段呈现出组织排列更有序的结构。接受3分钟光照处理的身体片段的情况与未受伤的片段非常相似。可以得出结论，在上述条件下用低功率激光处理时，再生质量会发生变化。
