Weiss N, Oron U
Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
Anat Embryol (Berl). 1992 Oct;186(5):497-503. doi: 10.1007/BF00185463.
The effect of low-energy laser (He-Ne) irradiation on the rate of skeletal muscle regeneration after partial excision of the rat gastrocnemius muscle was studied using quantitative histological morphometric methods. The injured zones of the experimental rats were subjected to direct He-Ne laser (632.8 nm wavelength) irradiation (6.0 mW for 2.3 min) immediately following injury and once daily thereafter for 5 days. Muscles that were injured as above and subjected to red or room light irradiation served as a control group. The volume fraction (percent of total volume of injured zone) of the mononucleated cells in the injured zone decreased gradually with time after injury, but more rapidly in the laser irradiated muscles than in the control. At 3 days post-injury the myotubes in the laser-irradiated muscles populated a significantly higher percentage (13.9 +/- 1.1%) of the injured area than in the control muscles (7.8 +/- 1.0%). The volume fraction of the young myofibers in the laser irradiated muscles exceeded 30.6 +/- 2.2% and 49.6 +/- 5.6% at 8 and 11 days post-injury, respectively, while in control muscles these structures comprised only 9.6 +/- 1.0% and 27.2 +/- 3.8% of the injured zone at 8 and 11 days after injury, respectively. It is concluded that He-Ne laser irradiation during the regeneration process promotes muscle maturation in the injured zone following partial excision of the rat gastrocnemius muscle.
采用定量组织学形态计量学方法，研究了低能量激光（氦氖）照射对大鼠腓肠肌部分切除后骨骼肌再生速率的影响。实验大鼠的损伤区域在受伤后立即接受直接的氦氖激光（波长632.8nm）照射（6.0mW，持续2.3分钟），此后每天照射一次，共照射5天。将上述受伤并接受红光或室内光照射的肌肉作为对照组。损伤区域中单核细胞的体积分数（占损伤区域总体积的百分比）在受伤后随时间逐渐降低，但激光照射组的肌肉中降低速度比对照组更快。在受伤后3天，激光照射组肌肉中的肌管在损伤区域中所占百分比（13.9±1.1%）显著高于对照组肌肉（7.8±1.0%）。在受伤后8天和11天，激光照射组肌肉中年轻肌纤维的体积分数分别超过30.6±2.2%和49.6±5.6%，而在对照组肌肉中，这些结构在受伤后8天和11天分别仅占损伤区域的9.6±1.0%和27.2±3.8%。得出的结论是，在大鼠腓肠肌部分切除后的再生过程中，氦氖激光照射可促进损伤区域的肌肉成熟。
