Lawaetz A J, Stedmon C A
Dept. Food Science, Faculty of Life Sciences, University Copenhagen, Rolighedsvej 30, DK-1958, Frederiksberg, Denmark.
Appl Spectrosc. 2009 Aug;63(8):936-40. doi: 10.1366/000370209788964548.
Fluorescence data of replicate samples obtained from different fluorescence spectrometers or by the same spectrometer but with different instrument settings can have great intensity differences. In order to compare such data an intensity calibration must be applied. Here we explain a simple calibration method for fluorescence intensity using only the integrated area of a water Raman peak. By applying this method to data from three different instruments, we show that it is possible to remove instrument-dependent intensity factors, and we present results on a unified scale of Raman units. The method presented is a rapid and simple approach suitable for routine measurements with no need for hazardous chemicals.
从不同荧光光谱仪或同一光谱仪但不同仪器设置获得的重复样品的荧光数据可能存在很大的强度差异。为了比较这些数据，必须进行强度校准。在这里，我们解释一种仅使用水拉曼峰的积分面积进行荧光强度校准的简单方法。通过将此方法应用于来自三种不同仪器的数据，我们表明可以消除仪器相关的强度因素，并在统一的拉曼单位尺度上呈现结果。所提出的方法是一种快速且简单的方法，适用于常规测量，无需使用危险化学品。
