Sakuma S, Ishikawa H, Okuma S
Department of Pediatrics, Jikei University School of Medicine, Tokyo, Japan.
Brain Res. 1990 Jan 8;506(2):307-10. doi: 10.1016/0006-8993(90)91268-l.
We examined the cell population of somatostatin (SS) in the periventricular nucleus (PN) and growth hormone-releasing factor (GRF) in the arcuate nucleus (ARC) between spontaneous dwarf rats (SDRs; gene symbol: dr), which show isolated GH deficiency, and normal rats using avidin-biotin complex (ABC) immunohistochemistry. The total number of SS perikarya per brain weight in the PN of SDRs was 824.8 +/- 49.6 (mean +/- S.E.M., n = 4), whereas that of controls was 1108.5 +/- 50.1 (n = 4). The GRF perikarya per brain weight in the ARC of SDRs numbered 1281.0 +/- 26.0 (n = 7), as compared to 685.4 +/- 64.6 (n = 7) in the controls. The SS perikarya in the PN of SDRs were significantly reduced (P less than 0.05), while the GRF perikarya in the SDRs were significantly increased (P less than 0.01). These results suggest that GH itself acts on SS to positively regulate its secretion and on GRF in a negative regulatory manner.
我们运用抗生物素蛋白-生物素复合物（ABC）免疫组织化学方法，对自发性侏儒大鼠（SDRs；基因符号：dr）和正常大鼠之间，室周核（PN）中生长抑素（SS）的细胞群以及弓状核（ARC）中生长激素释放因子（GRF）进行了研究。SDRs的PN中，每脑重的SS核周体总数为824.8±49.6（平均值±标准误，n = 4），而对照组为1108.5±50.1（n = 4）。SDRs的ARC中，每脑重的GRF核周体数量为1281.0±26.0（n = 7），相比之下，对照组为685.4±64.6（n = 7）。SDRs的PN中SS核周体显著减少（P < 0.05），而SDRs中GRF核周体显著增加（P < 0.01）。这些结果表明，生长激素本身对SS起作用，以正向调节其分泌，而对GRF则起负向调节作用。
