Gaetti-Jardim Elerson, Marcelino Silvia L, Feitosa Alfredo C R, Romito Giuseppe A, Avila-Campos Mario J
Anaerobe Laboratory, Department of Microbiology, University of São Paulo, São Paulo, SP, Brazil.
Laboratory of Molecular Pathology, Department of Periodontology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
J Med Microbiol. 2009 Dec;58(Pt 12):1568-1575. doi: 10.1099/jmm.0.013383-0. Epub 2009 Aug 13.
Oral pathogens, including periodontopathic bacteria, are thought to be aetiological factors in the development of cardiovascular disease. In this study, the presence of Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum-periodonticum-simiae group, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens and Tannerella forsythia in atheromatous plaques from coronary arteries was determined by real-time PCR. Forty-four patients displaying cardiovascular disease were submitted to periodontal examination and endarterectomy of coronary arteries. Approximately 60-100 mg atherosclerotic tissue was removed surgically and DNA was obtained. Quantitative detection of periodontopathic bacteria was performed using universal and species-specific TaqMan probe/primer sets. Total bacterial and periodontopathic bacterial DNA were found in 94.9 and 92.3 %, respectively, of the atheromatous plaques from periodontitis patients, and in 80.0 and 20.0 %, respectively, of atherosclerotic tissues from periodontally healthy subjects. All periodontal bacteria except for the F. nucleatum-periodonticum-simiae group were detected, and their DNA represented 47.3 % of the total bacterial DNA obtained from periodontitis patients. Porphyromonas gingivalis, A. actinomycetemcomitans and Prevotella intermedia were detected most often. The presence of two or more periodontal species could be observed in 64.1 % of the samples. In addition, even in samples in which a single periodontal species was detected, additional unidentified microbial DNA could be observed. The significant number of periodontopathic bacterial DNA species in atherosclerotic tissue samples from patients with periodontitis suggests that the presence of these micro-organisms in coronary lesions is not coincidental and that they may in fact contribute to the development of vascular diseases.
包括牙周病原菌在内的口腔病原体被认为是心血管疾病发生发展的病因。在本研究中，通过实时聚合酶链反应（PCR）测定了冠状动脉粥样斑块中伴放线聚集杆菌、具核梭杆菌 - 牙周亚种 - 猕猴亚种菌群、牙龈卟啉单胞菌、中间普氏菌、变黑普氏菌和福赛坦纳菌的存在情况。44例患有心血管疾病的患者接受了牙周检查和冠状动脉内膜切除术。手术切除约60 - 100毫克的动脉粥样硬化组织并提取DNA。使用通用和种特异性TaqMan探针/引物组对牙周病原菌进行定量检测。在牙周炎患者的动脉粥样斑块中，分别有94.9%和92.3%检测到总细菌DNA和牙周病原菌DNA；在牙周健康受试者的动脉粥样硬化组织中，这一比例分别为80.0%和20.0%。除具核梭杆菌 - 牙周亚种 - 猕猴亚种菌群外，所有牙周细菌均被检测到，其DNA占从牙周炎患者获得的总细菌DNA的47.3%。牙龈卟啉单胞菌、伴放线聚集杆菌和中间普氏菌的检出频率最高。64.1%的样本中可观察到两种或更多种牙周菌种的存在。此外，即使在仅检测到单一牙周菌种的样本中，也可观察到额外的未鉴定微生物DNA。牙周炎患者动脉粥样硬化组织样本中大量的牙周病原菌DNA种类表明，这些微生物在冠状动脉病变中的存在并非偶然，实际上它们可能有助于血管疾病的发展。
