Yang Xiao, Molimau Seeseei, Doherty Geoff P, Johnston Elecia B, Marles-Wright Jon, Rothnagel Rosalba, Hankamer Ben, Lewis Richard J, Lewis Peter J
School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia.
EMBO Rep. 2009 Sep;10(9):997-1002. doi: 10.1038/embor.2009.155. Epub 2009 Aug 14.
There are three stages of transcribing DNA into RNA. These stages are initiation, elongation and termination, and they are well-understood biochemically. However, despite the plethora of structural information made available on RNA polymerase in the last decade, little is available for RNA polymerase in complex with transcription elongation factors. To understand the mechanisms of transcriptional regulation, we describe the first structure, to our knowledge, for a bacterial RNA polymerase in complex with an essential transcription elongation factor. The resulting structure formed between the RNA polymerase and NusA from Bacillus subtilis provides important insights into the transition from an initiation complex to an elongation complex, and how NusA is able to modulate transcription elongation and termination.
将DNA转录为RNA有三个阶段。这些阶段是起始、延伸和终止，并且它们在生物化学上已得到充分理解。然而，尽管在过去十年中已有大量关于RNA聚合酶的结构信息，但关于与转录延伸因子结合的RNA聚合酶的信息却很少。为了理解转录调控机制，据我们所知，我们描述了第一个细菌RNA聚合酶与一种必需转录延伸因子结合的结构。枯草芽孢杆菌的RNA聚合酶与NusA之间形成的结构，为起始复合物向延伸复合物的转变，以及NusA如何调节转录延伸和终止提供了重要见解。
