Faculté Universitaire des Sciences Agronomiques de Gembloux, Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Passage des Déportés, 2, 5030 Gembloux, Belgium.
Appl Biochem Biotechnol. 2010 Sep;162(1):233-41. doi: 10.1007/s12010-009-8739-0. Epub 2009 Aug 13.
The production of gamma-decalactone and 4-hydroxydecanoic acid by the psychrophilic yeast R. aurantiaca was studied. The effect of both compounds on the growth of R. aurantiaca was also investigated and our results show that gamma-decalactone must be one of the limiting factors for its production. The addition of gum tragacanth to the medium at concentrations of 3 and 4 g/l seems to be an adequate strategy to enhance gamma-decalactone production and to reduce its toxicity towards the cell. The production of gamma-decalactone and 4-hydroxydecanoic acid was significantly higher in 20-l bioreactor than in 100-l bioreactor. By using 20 g/l of castor oil, 6.5 and 4.5 g/l of gamma-decalactone were extracted after acidification at pH 2.0 and distillation at 100 degrees C for 45 min in 20- and 100-l bioreactors, respectively. We propose a process at industrial scale using a psychrophilic yeast to produce naturally gamma-decalactone from castor oil which acts also as a detoxifying agent; moreover the process was improved by adding a natural gum.
我们研究了嗜冷酵母 R. aurantiaca 生产γ-癸内酯和 4-羟基癸酸的情况。还研究了这两种化合物对 R. aurantiaca 生长的影响，结果表明γ-癸内酯可能是其产生的限制因素之一。在培养基中添加 3 和 4 g/L 的黄蓍胶似乎是一种增强γ-癸内酯生产并降低其对细胞毒性的有效策略。在 20 升生物反应器中的生产γ-癸内酯和 4-羟基癸酸的量明显高于 100 升生物反应器。使用 20 g/L 的蓖麻油，在 pH 值为 2.0 时酸化并在 100°C 下蒸馏 45 分钟后，分别从 20 升和 100 升生物反应器中提取了 6.5 和 4.5 g/L 的γ-癸内酯。我们提出了一种在工业规模上使用嗜冷酵母从蓖麻油生产天然γ-癸内酯的工艺，蓖麻油同时也作为解毒剂；此外，通过添加天然胶，该工艺得到了改进。
