Centre Wallon de Biologie Industrielle, Gembloux, Belgium.
J Ind Microbiol Biotechnol. 2010 Feb;37(2):167-72. doi: 10.1007/s10295-009-0659-z. Epub 2009 Nov 10.
During the biotransformation of castor oil into gamma-decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of gamma-decalactone of 6.5 g/l was obtained. The parameters of gamma-decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of gamma-decalactone for the three Macronet resins were linear. The trapping of gamma-decalactone produced by R. aurantiaca on these resins was then carried out. gamma-Decalactone was effectively retained by all the studied Macronet resins. The resin MN-202 trapped gamma-decalactone more efficiently than MN-102 and MN-100. The percentages of gamma-decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed gamma-decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract gamma-decalactone from culture broth of R. aurantiaca.
在蓖麻油生物转化为γ-癸内酯的过程中，橙色粘帚霉同时产生了内酯形式和其前体（4-羟基癸酸）。经过六天的培养，获得了最大的γ-癸内酯产量为 6.5 g/l。在水中研究了三种 Macronet 树脂（MN-202、MN-102 和 MN-100）对γ-癸内酯的吸附参数。三种 Macronet 树脂对γ-癸内酯的吸附等温线呈线性。然后对橙色粘帚霉产生的γ-癸内酯在这些树脂上的捕集进行了研究。所有研究的 Macronet 树脂都有效地保留了γ-癸内酯。MN-202 树脂比 MN-102 和 MN-100 更有效地捕获γ-癸内酯。MN-202、MN-102 和 MN-100 树脂上吸附的γ-癸内酯的百分比分别为 85%、75%和 81%，而吸附的γ-癸内酯中有 70%左右被解吸。我们提出了一种使用 Macronet 树脂从橙色粘帚霉的发酵液中提取γ-癸内酯的工业工艺。
