Department of Food Science and Technology, University of Nebraska Lincoln, Lincoln, NE 68583-0919, USA.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Nov;25(11):1318-27. doi: 10.1080/02652030802132732.
The feasibility of applying a capillary zone electrophoretic (CZE) method for the trace analysis of bromate, a suspect human carcinogen, in drinking water was studied. Using a bare fused-silica capillary (75 microm inner diameter) coupled with indirect ultraviolet detection (200 nm), 0.25 mM cetyltrimethylammonium bromide, 5% methanol and 5.0 mM phthalate at pH 5.0-5.2, an electrokinetic injection of 15 kV and 10 s, a separation voltage of 18 kV (negative polarity) and a capillary temperature of 15 degrees C, bromate was detected in high purity water at < or =10 microg l(-1). The method was applied to three bottled water sources and to local municipal water. If needed, additional sample-handling steps, consisting of an off-line pre-concentration step and pH adjustment to 5.5, was used to improve detection limits (from a high of 400 microg l(-1) to < or =10 microg l(-1)) and baseline noise. Signal-to-noise ratios also increased by adding sodium phosphate (1.1 microg ml(-1)) to all sources prior to analysis. Although the CZE method was capable of detecting bromate at levels < or =10 microg l(-1) with an analysis time of 8-9 min, high variability (>10% relative standard deviation) precluded its application to some of sources without further method development. Nonetheless, this method could serve as the basis for the detection of bromate to specific water sources with minimal or no optimization.
研究了应用毛细管区带电泳（CZE）方法痕量分析饮用水中溴酸盐（一种可疑的人类致癌物）的可行性。使用裸熔融石英毛细管（75μm 内径），间接紫外检测（200nm），0.25mM 十六烷基三甲基溴化铵、5%甲醇和 5.0mM 邻苯二甲酸在 pH5.0-5.2 下，电动进样 15kV 和 10s，分离电压为 18kV（负极性），毛细管温度为 15°C，可以在高纯水中检测到<或=10μg l(-1)的溴酸盐。该方法应用于三种瓶装水源和当地市政用水。如果需要，还可以采用离线预浓缩步骤和 pH 调节至 5.5 等额外的样品处理步骤，以提高检测限（从高达 400μg l(-1)降至<或=10μg l(-1)）和基线噪声。在分析前向所有来源添加磷酸钠（1.1μg ml(-1))也可以提高信噪比。尽管 CZE 方法能够以 8-9 分钟的分析时间检测到<或=10μg l(-1)的溴酸盐，但由于高变异性（>10%相对标准偏差），在没有进一步方法开发的情况下，该方法无法应用于某些来源。尽管如此，该方法可以作为检测特定水源中溴酸盐的基础，无需或无需最小优化。
