Safavi Afsaneh, Maleki Norouz, Farjami Elaheh, Mahyari Farzaneh Aghakhani
Department of Chemistry, College of Sciences, and Nanotechnology Research Institute, Shiraz University, Shiraz, Iran.
Anal Chem. 2009 Sep 15;81(18):7538-43. doi: 10.1021/ac900501j.
Direct simultaneous electrochemical determination of glutathione (GSH) and glutathione disulfide (GSSG) has been presented using a nanoscale copper hydroxide carbon ionic liquid composite electrode. To the best of our knowledge, this is the first report on the simultaneous determination of these two biologically important compounds based on their direct electrochemical oxidation. Incorporation of copper(II) hydroxide nanostructures in the composite electrode results in complexation of Cu(II) with the thiol group of GSH and leads to a significant decrease in GSH oxidation overpotential, while an anodic peak corresponding to the direct oxidation of GSSG as the product of GSH oxidation is observed at higher overvoltages. Low detection limits of 30 nM for GSH and 15 nM for GSSG were achieved based on a signal-to-noise ratio of 3. The proposed method is free from interference of cysteine, homocysteine, ascorbic acid (AA), and uric acid (UA). No electrode surface fouling was observed during successive scans. Stability, high sensitivity, and low detection limits made the proposed electrode applicable for the analysis of biological fluids.
已报道使用纳米级氢氧化铜碳离子液体复合电极直接同时电化学测定谷胱甘肽（GSH）和谷胱甘肽二硫化物（GSSG）。据我们所知，这是基于这两种生物重要化合物的直接电化学氧化同时测定的首次报道。复合电极中氢氧化铜纳米结构的掺入导致Cu（II）与GSH的硫醇基团络合，并导致GSH氧化过电位显著降低，而在较高过电位下观察到对应于作为GSH氧化产物的GSSG直接氧化的阳极峰。基于3的信噪比，GSH的检测限低至30 nM，GSSG的检测限低至15 nM。所提出的方法不受半胱氨酸、同型半胱氨酸、抗坏血酸（AA）和尿酸（UA）的干扰。在连续扫描过程中未观察到电极表面污染。稳定性、高灵敏度和低检测限使得所提出的电极适用于生物流体的分析。
