Federal State Budget Educational Institution of Higher Education, "MIREA-Russian Technological University", Vernadsky Avenue 86, Moscow, 119454, Russia.
Semenov Institute of Chemical Physics of Russian Academy of Sciences, Kosygina street 4, Moscow, 4119991, Russia.
Amino Acids. 2022 Jun;54(6):911-922. doi: 10.1007/s00726-022-03166-0. Epub 2022 May 18.
The simple and facilitated transfer of tripeptide glutathione across the water/2-nitrophenyl octhyl ether interface was studied via cyclic voltammetry at interface between two immiscible electrolyte solutions (ITIES). The micro-perforated membrane prepared with a laser with a femtosecond pulse was used for mechanical stabilization of the interface. The method of cyclic voltammetry was used to study the passive and facilitated interfacial transfer of glutathione and its complex with the crown ether dibenzo-18-crown-6 (DB18C6).The glutathione mass transfer mechanism was established and substantiated, the diffusion coefficients, thermodynamic characteristics of interphase transfer and the constant of complexation of the glutathione by DB18C6 were determined. Square wave voltammetry based on facilitated transfer was used for more accurate and sensitive determination of glutathione low detection limit (0.8 μM) with wide linear dynamic range (from 3.0 to 80 μM) was reached. The influence of various potentially interfering ions on the voltammetric determination of glutathione has also been investigated. The method developed was applied to determine glutathione in aqueous solutions and malt extract.
通过在两不相容电解质溶液（ITIES）之间的界面处使用循环伏安法，研究了三肽谷胱甘肽在水/2-硝基苯辛基醚界面处的简单且易于传递。使用飞秒脉冲激光制备的微孔膜用于机械稳定界面。使用循环伏安法研究了谷胱甘肽及其与冠醚二苯并-18-冠-6（DB18C6）的配合物的被动和促进的界面传递。建立并证实了谷胱甘肽的传质机制，确定了扩散系数、相间传递的热力学特性以及 DB18C6 与谷胱甘肽的配合常数。基于促进传递的方波伏安法用于更准确和灵敏地测定谷胱甘肽，检测限低（0.8 μM），线性动态范围宽（从 3.0 到 80 μM）。还研究了各种潜在干扰离子对谷胱甘肽伏安测定的影响。开发的方法用于测定水溶液和麦芽提取物中的谷胱甘肽。
