Institute of Pathological Physiology, Center of Experimental Hematology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
Physiol Res. 2010;59(3):393-400. doi: 10.33549/physiolres.931759. Epub 2009 Aug 12.
Hepcidin, a key regulator of iron metabolism, plays a crucial role in the pathogenesis of anemia of chronic disease. Although it is produced mainly in the liver, its recently described expression in adipose tissue has been shown to be enhanced in massive obesity due to chronic low-grade inflammation. Our objective was to study the changes in hepcidin expression in adipose tissue during acute-phase reaction. We measured hepcidin mRNA expression from isolated subcutaneous and epicardial adipose tissue at the beginning and at the end of the surgery. The expression of mRNAs for hepcidin and other iron-related genes (transferrin receptor 1, divalent metal transporter 1, ferritin, ferroportin) were measured by real-time RT-PCR. Hepcidin expression significantly increased at the end of the surgery in subcutaneous but not in epicardial adipose tissue. Apart from the increased levels of cytokines, the parameters of iron metabolism showed typical inflammation-induced changes. We suggest that acute inflammatory changes could affect the regulation of hepcidin expression in subcutaneous adipose tissue and thus possibly contribute to inflammation-induced systemic changes of iron metabolism.
铁调素是铁代谢的关键调节剂，在慢性病性贫血的发病机制中起着至关重要的作用。尽管铁调素主要在肝脏中产生，但最近的研究表明，在肥胖症患者中，由于慢性低度炎症，脂肪组织中铁调素的表达会增强。我们的目的是研究在急性期反应过程中脂肪组织中铁调素表达的变化。我们在手术开始和结束时测量了分离的皮下和心外膜脂肪组织中铁调素 mRNA 的表达。通过实时 RT-PCR 测量铁调素和其他铁相关基因（转铁蛋白受体 1、二价金属转运蛋白 1、铁蛋白、亚铁转运蛋白）的 mRNA 表达。手术结束时，皮下脂肪组织中铁调素的表达显著增加，但心外膜脂肪组织中没有增加。除了细胞因子水平升高外，铁代谢参数也表现出典型的炎症诱导变化。我们认为急性炎症变化可能会影响皮下脂肪组织中铁调素表达的调节，从而可能导致炎症诱导的全身铁代谢变化。
