早发型重度子痫前期母血基因表达谱分析：新型生物标志物的鉴定。
Gene expression profiling of maternal blood in early onset severe preeclampsia: identification of novel biomarkers.
机构信息
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, PR China.
出版信息
J Perinat Med. 2009;37(6):609-16. doi: 10.1515/JPM.2009.103.
AIMS
To investigate candidate genes in peripheral blood mononuclear cell (PBMC) that are associated with early onset severe preeclampsia (ES-PE) and to describe candidate genes function using microarrays and real-time polymerase chain reaction (PCR).
METHODS
PBMC RNA was extracted from six patients with ES-PE and five uncomplicated pregnancies. The HG_U133 plus 2.0 Affymetrix GeneChips that represented 47,000 genes were used to measure gene expression in each sample. Significance analysis of microarray identified potential signature genes characterizing ES-PE vs. uncomplicated pregnancies. Eight genes were selected for confirmation by real-time PCR of 32 patients with ES-PE and 24 uncomplicated pregnancies, matched for maternal age, parity, race and gestational weeks.
RESULTS
Using a whole-genome approach to study the molecular determinants of ES-PE, 72 genes were found to be differentially expressed between cases and controls, including 38 up-regulated genes and 34 down-regulated genes in the group of ES-PE. Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2 (KIR3DL2), aldo-keto reductase family 1, member C3 (AKR1C3), churchill domain containing 1 (CHURC1), and solute carrier family 25, member 13 (SLC25A13) were validated to be down-regulated in the patients with ES-PE by real-time PCR.
CONCLUSIONS
Expression of genes with diverse function is associated with ES-PE risk, providing opportunities for the development of non-invasive diagnosis.
目的
研究与早发型重度子痫前期（ES-PE）相关的外周血单个核细胞（PBMC）中的候选基因，并使用微阵列和实时聚合酶链反应（PCR）描述候选基因的功能。
方法
从 6 例 ES-PE 患者和 5 例非复杂妊娠患者中提取 PBMC RNA。使用代表 47,000 个基因的 HG_U133 plus 2.0 Affymetrix GeneChips 测量每个样本中的基因表达。微阵列的显著性分析确定了潜在的特征基因，这些基因可将 ES-PE 与非复杂妊娠区分开来。选择 8 个基因通过实时 PCR 在 32 例 ES-PE 患者和 24 例非复杂妊娠患者中进行验证，这些患者的母亲年龄、产次、种族和妊娠周数相匹配。
结果
使用全基因组方法研究 ES-PE 的分子决定因素，发现 72 个基因在病例组和对照组之间表达差异，其中 ES-PE 组有 38 个上调基因和 34 个下调基因。杀伤细胞免疫球蛋白样受体，三个结构域，长胞质尾，2（KIR3DL2），醛酮还原酶家族 1，成员 C3（AKR1C3），丘吉尔结构域包含 1（CHURC1）和溶质载体家族 25，成员 13（SLC25A13）通过实时 PCR 验证在 ES-PE 患者中下调。
结论
具有不同功能的基因表达与 ES-PE 风险相关，为非侵入性诊断的发展提供了机会。
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