Kuzir S, Kozarić Z, Gjurcević E, Bazdarić B, Petrinec Z
Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
Anat Histol Embryol. 2009 Oct;38(5):351-4. doi: 10.1111/j.1439-0264.2009.00953.x. Epub 2009 Aug 13.
Garfish, Belone belone (Linnaeus, 1761) is an elongate, slander fish inhabiting the Eastern Atlantic and Mediterranean Sea. These fish from the Belonidae (Actinopterygii) family have important commercial value for Croatian fisheries. Samples for research were collected from experimental hatching in the Novigrad Sea. Preparation techniques included fixation in buffered formalin, trypsin clearing and staining with alcian blue and alizarin red. As little is known of garfish osteology and bone morphology, the main goal of this study was to describe ossification process in garfish fry. At hatching, no skeletal structure is present. Newly-hatched larvae also had no osteological elements. Ossification started at 7 day post-hatching (DPH) [total length (TL) 18 mm] with head bones and vertebral neural arch. Head skeleton continued to develop mostly over the period from 7 to 10 DPH. At 21 DPH (TL 49 mm), ossification process seemed to be finished, but it was not possible to distinguish borders of all bones. The primary interest of our research was to understand the growth dynamics as well as transformation of supporting body elements from cartilage to bone. At the end, developmental characteristics and functional aspects of this formation in different fish species are discussed.
长颌鱼，Belone belone（林奈，1761年）是一种细长的鱼类，栖息于东大西洋和地中海。这些来自颌针鱼科（辐鳍鱼纲）的鱼类对克罗地亚渔业具有重要的商业价值。研究样本取自诺维格拉德海域的实验性孵化。制备技术包括用缓冲福尔马林固定、胰蛋白酶透明处理以及用阿尔新蓝和茜素红染色。由于对长颌鱼的骨学和骨骼形态了解甚少，本研究的主要目的是描述长颌鱼苗的骨化过程。孵化时，不存在骨骼结构。刚孵化的幼体也没有骨学元素。骨化始于孵化后7天（DPH）[全长（TL）18毫米]，首先是头骨和椎骨神经弓。头部骨骼主要在7至10 DPH期间持续发育。在21 DPH（TL 49毫米）时，骨化过程似乎完成，但无法区分所有骨骼的边界。我们研究的主要兴趣在于了解生长动态以及支持身体的元素从软骨到骨骼的转变。最后，讨论了不同鱼类物种中这种形成的发育特征和功能方面。
