Department of Biological and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland.
Photochem Photobiol. 2009 Nov-Dec;85(6):1346-55. doi: 10.1111/j.1751-1097.2009.00597.x.
In research concerning stratospheric ozone depletion, action spectra are used as biological spectral weighting functions (BSWFs) for describing the effects of UV radiation on plant responses. Our aim was to evaluate the appropriateness of six frequently used BSWFs that differ in effectiveness with increasing wavelength. The evaluation of action spectra was based on calculating the effective UV radiation doses according to 1-2) two formulations of the generalized plant action spectrum, 3) a spectrum for ultraviolet induced erythema in human skin, 4) a spectrum for the accumulation of a flavonol in Mesembryanthemum crystallinum, 5) a spectrum for DNA damage in alfalfa seedlings and 6) the plant growth action spectrum. We monitored effects of UV radiation on the concentration of individual UV absorbing metabolites and chlorophyll concentrations in leaves and growth responses of silver birch (Betula pendula) seedlings. Experiments were conducted outdoors using plastic films attenuating different parts of the UV spectrum. Chlorophyll concentrations and growth were not affected by the UV treatments. The response to UV radiation varied between and within groups of phenolics. In general, the observed responses of phenolic groups and individual flavonoids were best predicted by action spectra extending into the UV-A region with moderate effectiveness.
在研究平流层臭氧消耗时，作用光谱被用作描述紫外线辐射对植物反应影响的生物光谱加权函数（BSWF）。我们的目的是评估六种常用的 BSWF 的适宜性，这些 BSWF 在有效性上随波长的增加而不同。作用光谱的评估是基于根据广义植物作用光谱的两种公式 1-2）、人类皮肤紫外线诱导红斑的光谱 3）、在 Mesembryanthemum crystallinum 中积累类黄酮的光谱 4）、紫花苜蓿幼苗 DNA 损伤的光谱 5）和植物生长作用光谱 6）计算有效紫外线剂量来进行的。我们监测了紫外线辐射对叶片中个别紫外线吸收代谢物浓度和叶绿素浓度以及银桦（Betula pendula）幼苗生长反应的影响。实验在户外进行，使用了衰减紫外线光谱不同部分的塑料薄膜。叶绿素浓度和生长不受紫外线处理的影响。酚类物质之间和内部的紫外线反应存在差异。一般来说，观察到的酚类物质和个别类黄酮的反应最好由延伸到 UV-A 区域的作用光谱来预测，其有效性中等。
