Laboratório de Neurociências and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil.
Neurochem Int. 2009 Dec;55(8):802-5. doi: 10.1016/j.neuint.2009.08.003. Epub 2009 Aug 12.
Dystrophin is a protein found at the plasmatic membrane in muscle and postsynaptic membrane of some neurons, where it plays an important role on synaptic transmission and plasticity. Its absence is associated with Duchenne's muscular dystrophy (DMD), in which cognitive impairment is found. Oxidative stress appears to be involved in the physiopathology of DMD and its cognitive dysfunction. In this regard, the present study investigated oxidative parameters (lipid and protein peroxidation) and antioxidant enzymes activities (superoxide dismutase and catalase) in prefrontal cortex, cerebellum, hippocampus, striatum and cortex tissues from male dystrophic mdx and normal C57BL10 mice. We observed (1) reduced lipid peroxidation in striatum and protein peroxidation in cerebellum and prefrontal cortex; (2) increased superoxide dismutase activity in cerebellum, prefrontal cortex, hippocampus and striatum; and (3) reduced catalase activity in striatum. It seems by our results, that the superoxide dismutase antioxidant mechanism is playing a protective role against lipid and protein peroxidation in mdx mouse brain.
肌营养不良蛋白是一种存在于肌肉质膜和某些神经元突触后膜的蛋白质，它在突触传递和可塑性中起着重要作用。其缺失与杜氏肌营养不良症（DMD）有关，其中发现认知障碍。氧化应激似乎与 DMD 的病理生理学及其认知功能障碍有关。在这方面，本研究调查了前额叶皮层、小脑、海马、纹状体和皮质组织中氧化参数（脂质和蛋白质过氧化）和抗氧化酶活性（超氧化物歧化酶和过氧化氢酶）在雄性肌营养不良症 mdx 和正常 C57BL10 小鼠中的情况。我们观察到：（1）纹状体中的脂质过氧化减少，小脑和前额叶皮层中的蛋白质过氧化增加；（2）小脑、前额叶皮层、海马和纹状体中的超氧化物歧化酶活性增加；（3）纹状体中的过氧化氢酶活性降低。根据我们的结果，似乎超氧化物歧化酶抗氧化机制在 mdx 小鼠大脑中对脂质和蛋白质过氧化起到了保护作用。
