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经内镜处理的体外和体内胚胎发育方法:牛输卵管的应用。

Endoscopic approaches to manage in vitro and in vivo embryo development: use of the bovine oviduct.

机构信息

Reproduction Center Wieselburg, University of Veterinary Medicine, Vienna, Austria.

出版信息

Theriogenology. 2010 Apr 1;73(6):768-76. doi: 10.1016/j.theriogenology.2009.07.003. Epub 2009 Aug 13.

Abstract

The oviduct plays a major part in different reproductive processes providing the microenvironment for numerous steps in early embryogenesis. Consequently, there is a growing demand to perform comparative studies focusing on causal mechanisms related to embryo development within its environment including complex and holistic strategies. However, the routine flushing and transfer procedure of bovine embryos is limited to the morula and blastocyst stage. Additionally, the use of in vitro production of bovine embryos provides access to an extra amount of embryos at various stages. But the quality of these embryos does not reflect the quality of its ex vivo counterparts. For two decades our own studies have focused on use of the oviductal environment of different species to optimize early embryo development for different purposes. The current article briefly highlights some main characteristics of the fallopian tube and reviews the endoscopic approach to access the fallopian tube using the stepwise minimal invasive technique established in different species.

摘要

输卵管在不同的生殖过程中起着重要作用,为早期胚胎发生的许多步骤提供了微环境。因此,人们越来越需要进行专注于与胚胎发育环境相关的因果机制的比较研究,包括复杂和整体的策略。然而,牛胚胎的常规冲洗和转移程序仅限于桑椹胚和囊胚阶段。此外,体外生产牛胚胎的方法可以获得不同阶段的大量胚胎。但这些胚胎的质量并不能反映其离体对应物的质量。二十年来,我们的研究重点一直是利用不同物种的输卵管环境来优化不同目的的早期胚胎发育。本文简要介绍了输卵管的一些主要特征,并回顾了使用在不同物种中建立的逐步微创技术进入输卵管的内镜方法。

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