Awad J, Ollier W, Cutbush S, Papasteriadis C, Gupta A, Carthy D, McCloskey D, Brown C J, Boki K, Fostizopoulos G
Department of Immunology, London Hospital, Medical College, U.K.
Tissue Antigens. 1990 Jan;35(1):40-4. doi: 10.1111/j.1399-0039.1990.tb01753.x.
It has been shown that Greek RA patients do not show an increased frequency of HLA-DR4 compared with Greek controls. As only the Dw4 and Dw14 subtypes of DR4 are implicated in RA, we have characterised DR4-positive Greek RA patients and controls using serology, MLC typing and RFLP analysis to see whether the distribution of DR4 subtypes or other HLA antigens associated with DR4 could account for these findings. In the 10 patients and 12 controls studied, Dw10 was common, being present in almost half the controls. Dw4 was not detected in the controls, but was present in three of the RA patients, indicating that there may be some relationship between Dw4 and RA in this population; Dw13 and Dw14 were also found, Dw15 was not detected. Eight of the subjects did not type as any of the HLA-D antigens mentioned. Three controls had unusual DR4-DQ associations, two having DR4-DQw2 and one possessing DR4-DQw4. Analysis of the TaqI DRB RFLP of the subjects showed that one control did not have the 6.1 kb band characteristic of DR4s. All these results indicate that DR4 is a heterogeneous antigen in this population and that several as yet undescribed variants of DR4 may be present.
研究表明，与希腊对照组相比，希腊类风湿关节炎（RA）患者中HLA - DR4的频率并未增加。由于只有DR4的Dw4和Dw14亚型与RA有关，我们采用血清学、混合淋巴细胞培养（MLC）分型和限制性片段长度多态性（RFLP）分析对DR4阳性的希腊RA患者和对照组进行了特征分析，以确定DR4亚型或与DR4相关的其他HLA抗原的分布是否可以解释这些结果。在所研究的10例患者和12例对照中，Dw10很常见，几乎在一半的对照中出现。在对照中未检测到Dw4，但在3例RA患者中存在，这表明在该人群中Dw4与RA之间可能存在某种关系；也发现了Dw13和Dw14，未检测到Dw15。8名受试者未能分型为上述任何一种HLA - D抗原。3名对照具有不寻常的DR4 - DQ关联，2名具有DR4 - DQw2，1名具有DR4 - DQw4。对受试者的TaqI DRB RFLP分析表明，1名对照不具有DR4特有的6.1 kb条带。所有这些结果表明，在该人群中DR4是一种异质性抗原，可能存在几种尚未描述的DR4变体。
