Ollier W, Carthy D, Cutbush S, Okoye R, Awad J, Fielder A, Silman A, Festenstein H
Department of Immunology, London Hospital Medical College, U.K.
Tissue Antigens. 1989 Jan;33(1):30-7. doi: 10.1111/j.1399-0039.1989.tb01674.x.
Frequencies of HLA-DR4 and its related Dw types were compared between randomly selected normal controls and the index cases of multiplex rheumatoid arthritis (RA) families. A DR4 frequency of 68.3% was observed in index cases (n = 57) compared to 31.2% in normal controls (n = 96). Cellular typing with homozygous typing cells (HTCs) revealed significant increases of Dw4 (49.1% vs 22.9% RR = 3.2 p less than 0.001) and Dw14 (22.8% vs 2.1% RR = 13.9 p less than 0.001) in the index cases. A non-significant increase was seen for Dw13 (8.8% vs 4.1%). When DR4 positive patients and controls were compared, a significant increase was seen only for Dw14 (34.2% vs 6.6% RR = 7.3 p less than 0.01). Data from HLA genotyped RA and normal families allowed an examination of haplotype combinations of HLA-B antigens and DR4/Dw types to be made. HLA-Dw4 was predominantly found with B44 and Bw62 with nearly all DR4/Bw62 haplotypes being Dw4 positive. HLA-Dw13 was associated with B44 and Dw14 with Bw60, B44 and B27. Based on HTC and normal family data. Dw10 was found to be strongly associated with B38 containing haplotypes. Analysis of 69 C4A, C4B complement typed DR4 haplotypes failed to show any statistically significant association between Dw type and "complotype". However, there was a suggestion of C4A3. BQO being associated with Dw4 (34.2% vs 16.1% X2 = 2.9 p = ns) and C4A3, B1 with Dw14 (45.5% vs 27.6% X2 = 2.1 p = ns).(ABSTRACT TRUNCATED AT 250 WORDS)
在随机选取的正常对照与多发型类风湿关节炎（RA）家系的索引病例之间，比较了HLA - DR4及其相关Dw类型的频率。索引病例（n = 57）中观察到DR4频率为68.3%，而正常对照（n = 96）中为31.2%。用纯合分型细胞（HTC）进行细胞分型显示，索引病例中Dw4（49.1%对22.9%，相对风险率RR = 3.2，p小于0.001）和Dw14（22.8%对2.1%，RR = 13.9，p小于0.001）显著增加。Dw13有非显著增加（8.8%对4.1%）。当比较DR4阳性患者和对照时，仅Dw14有显著增加（34.2%对6.6%，RR = 7.3，p小于0.01）。来自HLA基因分型的RA和正常家系的数据使得能够对HLA - B抗原与DR4/Dw类型的单倍型组合进行检查。HLA - Dw4主要与B44和Bw62一起被发现，几乎所有DR4/Bw62单倍型都是Dw4阳性。HLA - Dw13与B44相关，Dw14与Bw60、B44和B27相关。基于HTC和正常家系数据，发现Dw10与含B38的单倍型强烈相关。对69个C4A及C4B补体分型的DR4单倍型的分析未能显示Dw类型与“补体型”之间有任何统计学上的显著关联。然而，有迹象表明C4A3.BQ0与Dw4相关（34.2%对16.1%，X2 = 2.9 p =无统计学意义），C4A3.B1与Dw14相关（45.5%对27.6%，X2 = 2.1 p =无统计学意义）。（摘要截短于250字）
