Institute of Experimental Pathology and Parasitology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bl. 25, 1113 Sofia, Bulgaria.
Eur J Med Chem. 2009 Dec;44(12):5099-102. doi: 10.1016/j.ejmech.2009.07.016. Epub 2009 Jul 22.
The biological activity of previously synthesized compounds [(phen)(3)(H(+))(2)(NO(3)(-))(2) (1), Pd(5-NH(2)-phen)(2)(NO(3))(2) (2) and Pd(phen)(2)(NO(3))(2)(H(2)O) (3)] was investigated in vivo. The three compounds did not show any histological alterations in the observed lung, liver, spleen and lymph nodes of White Wistar rats. The propidium iodine staining did not discover any cytotoxic effect of the tested derivatives. The tests for immunological response predominantly showed stimulation of the antibody-producing B-cells and lower or no stimulation of the T-cells. The LIF-test showed better stimulation of all lymphocytes with 1, followed by 2 and 3. Substance 3 showed highest stimulating effect on B-cell blood lymphocytes in all doses (maximum in the lowest dose), whereas the impact of 2 is weaker and that of 1 is the weakest. The T-cell immune response after treatment with substance 1 is best influenced by dose of 1mg in the spleen cell-fraction, followed by 3 (5 mg).
研究了先前合成的化合物 [(phen)(3)(H(+))(2)(NO(3)(-))(2) (1), Pd(5-NH(2)-phen)(2)(NO(3))(2) (2) 和 Pd(phen)(2)(NO(3))(2)(H(2)O) (3)] 的体内生物活性。这三种化合物在观察到的白 Wistar 大鼠的肺、肝、脾和淋巴结中没有显示出任何组织学改变。碘化丙啶染色未发现测试衍生物有任何细胞毒性作用。免疫反应测试主要显示出对产生抗体的 B 细胞的刺激作用，而对 T 细胞的刺激作用较低或没有。LIF 测试显示，1 对所有淋巴细胞的刺激作用最好，其次是 2 和 3。在所有剂量下，化合物 3 对 B 细胞血液淋巴细胞的刺激作用最强（在最低剂量下达到最大值），而 2 的作用较弱，1 的作用最弱。在脾细胞部分中，用 1mg 剂量处理后，1 对 T 细胞免疫反应的影响最佳，其次是 3（5mg）。
