Schubert T, Müller W E
Department of Psychopharmacology, Central Institute of Mental Health, Mannheim, Federal Republic of Germany.
Biochem Pharmacol. 1990 Feb 1;39(3):439-44. doi: 10.1016/0006-2952(90)90048-p.
The presence of guanylate cyclase in intact circulating human lymphocytes and the sensitivity of this enzyme to stimulation by sodium nitroprusside could be confirmed. However, in contrast to other observations all attempts failed to stimulate the enzyme by cholinergic agonists, despite the use of M1 as well as M2 selective agonists. These findings do not support the assumption that cholinergic recognition sites on human lymphocytes described by many groups are part of a functioning muscarinic transducing mechanism. While several other neuroreceptor agonists were also unable to affect lymphocyte guanylate cyclase activity, lithium was found to potently inhibit the stimulation of guanylate cyclase by sodium nitroprusside at an intracellular concentration close to the therapeutic plasma levels. It is suggested that the effects of lithium on guanylate cyclase activity in human lymphocytes could be related to a possible mechanism of action of lithium in affective disorders.
可以证实，完整的循环人类淋巴细胞中存在鸟苷酸环化酶，且该酶对硝普钠刺激敏感。然而，与其他观察结果相反，尽管使用了M1和M2选择性激动剂，但所有试图通过胆碱能激动剂刺激该酶的尝试均告失败。这些发现不支持许多研究小组所描述的人类淋巴细胞上的胆碱能识别位点是功能性毒蕈碱转导机制一部分的假设。虽然其他几种神经受体激动剂也无法影响淋巴细胞鸟苷酸环化酶活性，但发现锂在细胞内浓度接近治疗性血浆水平时能有效抑制硝普钠对鸟苷酸环化酶的刺激。有人认为，锂对人类淋巴细胞鸟苷酸环化酶活性的影响可能与锂在情感障碍中的一种可能作用机制有关。
