García-Peña E I, Guerrero-Barajas C, Ramirez D, Arriaga-Hurtado L G
Bioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología-IPN, Av., Acueducto s/n. P.O. Box 07340, DF, Mexico.
Bioresour Technol. 2009 Dec;100(24):6369-77. doi: 10.1016/j.biortech.2009.07.033. Epub 2009 Aug 14.
In this work, a semi-continuous biological system was established to produce hydrogen and generate electricity by coupling the bioreactor to a fuel cell. Heat and acid pretreatments (at 35 and 55 degrees C) of a seed sludge used as inoculum were performed in order to increase hydrogen producers. Different initial glucose concentrations (IGC) were tested for heat pretreated inoculum at 35 degrees C to determine the optimum concentration of glucose that supported the highest hydrogen production. Results showed that the heat pretreated inoculums (35 degrees C) reached the highest hydrogen molar yield of 2.85 mol H(2)/mol glucose (0.014 L/h), which corresponds to the acetic acid pathway. At the optimum IGC (10 g/L, 35 degrees C) the hydrogen molar yield was 3.6 mol H(2)/mol glucose (0.023 L/h). The coupled bioreactor-fuel cell system yielded an output voltage of 1.06 V, power of 0.1 W (25 degrees C) and a current of 68 mA. The overall results suggest that high hydrogen molar yields can be obtained through the acetic acid pathway and that is feasible to generate electricity using hydrogen from the semi-continuous bioreactor.
在这项工作中，通过将生物反应器与燃料电池耦合，建立了一个半连续生物系统来制氢和发电。对用作接种物的种子污泥进行了热预处理和酸预处理（分别在35和55摄氏度下），以增加产氢菌。对在35摄氏度下经过热预处理的接种物测试了不同的初始葡萄糖浓度（IGC），以确定支持最高产氢量的最佳葡萄糖浓度。结果表明，经过热预处理的接种物（35摄氏度）达到了最高的氢摩尔产率，为2.85摩尔H₂/摩尔葡萄糖（0.014升/小时），这对应于乙酸途径。在最佳IGC（10克/升，35摄氏度）下，氢摩尔产率为3.6摩尔H₂/摩尔葡萄糖（0.023升/小时）。生物反应器-燃料电池耦合系统产生的输出电压为1.06伏，功率为0.1瓦（25摄氏度），电流为68毫安。总体结果表明，通过乙酸途径可以获得高氢摩尔产率，并且利用半连续生物反应器产生的氢气发电是可行的。
