Wei Daqing, Zhou Yu, Yang Chunhui
School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, PR China.
Colloids Surf B Biointerfaces. 2009 Nov 1;74(1):230-7. doi: 10.1016/j.colsurfb.2009.07.025. Epub 2009 Jul 25.
Chemical treatment was used to modify the surface of microarc oxidized (MAO) coating containing Ca and P. And the chemically treated MAO (C-MAO) coating was further heat-treated at 400-800 degrees C. The average roughness of the MAO and C-MAO coatings is about 250 nm; further heat treatment improved the roughness of the C-MAO coating. The chemical and heat treatment enhanced the wetting ability of the MAO coating. During SBF immersion, amorphous Ca- and P-containing precipitate (Ca-P) appeared firstly, eventually transforming to crystalline apatites. At the same time, the apatite firstly formed at the concave region on the surface, and then spreads on the whole surface. The induced biomimetic apatite possessed a porous structure on two-scales of micron and nanometer levels. In the interlayer and outlayer of the C-MAO coating, the elements of Ca, P and Ti showed a gradient distribution. In the apatite layer, Ti disappeared basically, and the concentrations of Ca and P did not change obviously. In this work, continuous MG63 cell layer was observed on the surface of the C-MAO coating, which can provide good environment for the cell proliferation.
采用化学处理方法对含Ca和P的微弧氧化（MAO）涂层表面进行改性。对化学处理后的MAO（C-MAO）涂层进一步在400-800℃下进行热处理。MAO和C-MAO涂层的平均粗糙度约为250nm；进一步热处理提高了C-MAO涂层的粗糙度。化学处理和热处理增强了MAO涂层的润湿性。在模拟体液（SBF）浸泡过程中，首先出现含无定形Ca和P的沉淀物（Ca-P），最终转变为结晶磷灰石。同时，磷灰石首先在表面的凹坑区域形成，然后扩展到整个表面。诱导形成的仿生磷灰石在微米和纳米两个尺度上均具有多孔结构。在C-MAO涂层的中间层和外层，Ca、P和Ti元素呈梯度分布。在磷灰石层中，Ti基本消失，Ca和P的浓度没有明显变化。在这项工作中，在C-MAO涂层表面观察到连续的MG63细胞层，这可以为细胞增殖提供良好的环境。
