Cui Shenghui, Li Jingyun, Hu Changqin, Jin Shaohong, Li Fengqin, Guo Yunchang, Ran Lu, Ma Yue
The State Food and Drug Administration, Beijing, People's Republic of China.
J Antimicrob Chemother. 2009 Oct;64(4):680-3. doi: 10.1093/jac/dkp275. Epub 2009 Aug 14.
The objectives of this study were to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in livestock and related workers in four Chinese provinces and the characteristics of these isolates.
Nasal swabs were collected from animals and farm workers in four Chinese provinces. MRSA isolates were recovered and characterized by PFGE, Panton-Valentine leucocidin PCR, staphylococcal chromosomal cassette (SCC) mec typing, spa typing, multilocus sequence typing, antimicrobial susceptibility testing and testing for inducible clindamycin resistance.
A total of 60 MRSA isolates were recovered from swine and swine workers. Two predominant multidrug resistance profiles were identified: ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline/chloramphenicol and ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline. All isolates were determined to be spa type t899, contained the group III SCCmec element and were Panton-Valentine leucocidin negative. Multilocus sequence type ST9 (n = 46) was identified as the dominant sequence type. One dominant PFGE cluster and a dominant strain type were identified.
MRSA from Chinese pigs and farm workers (ST9) differed from the European pig-associated clone (ST398) with regard to clonal type, SCCmec content and resistance profile.
本研究旨在确定中国四个省份家畜及相关从业人员中甲氧西林耐药金黄色葡萄球菌（MRSA）的定植率及其分离株的特征。
采集中国四个省份动物和农场工人的鼻拭子。通过脉冲场凝胶电泳（PFGE）、杀白细胞素PCR、葡萄球菌染色体盒式（SCC）mec分型、spa分型、多位点序列分型、抗菌药物敏感性试验及诱导型克林霉素耐药性检测对MRSA分离株进行鉴定和特征分析。
共从猪及养猪工人中分离出60株MRSA。确定了两种主要的多重耐药谱：环丙沙星/克林霉素/红霉素/头孢西丁/庆大霉素/四环素/氯霉素耐药谱和环丙沙星/克林霉素/红霉素/头孢西丁/庆大霉素/四环素耐药谱。所有分离株均确定为spa型t899，含有III组SCCmec元件，杀白细胞素阴性。多位点序列分型确定ST9型（n = 46）为主要序列型。确定了一个主要的PFGE簇和一个主要菌株型。
来自中国猪和农场工人的MRSA（ST9型）在克隆类型、SCCmec含量和耐药谱方面与欧洲猪相关克隆（ST398型）不同。
