Moraes J R E, Facco G G, Moraes F R, Engracia Filho J R, Miyazato L G, Beretta D C
Department of Veterinary Pathology, Faculty of Agricultural and Veterinary Sciences, São Paulo State University, Via de Accesso Prof Paulo Donato Castellane s/n, Jaboticabal, CEP 14884-900, SP, Brazil.
Vet Rec. 2009 Aug 15;165(7):203-5. doi: 10.1136/vr.165.7.203.
An inflammatory process was induced by intratendinous injection of bacterial collagenase into the superficial digital flexor tendon (SDFT) of the left thoracic limb of 10 horses. One week later, the tendons in five of the horses (group 1) were treated with glycosaminoglycan polysulphate (GAGPS), and the tendons of the other five (group 2) were treated with saline solution. The horses were euthanased 150 days after the collagenase injections, and samples of the SDFTs were frozen at -14 degrees C, sectioned at 5 to 7 mum longitudinally and transversely, and stained by the picrosirius red method. Morphometric analysis was used to quantify the organised and disorganised bundles of collagen in the samples from groups 1 and 2. Significantly more organised bundles of collagen were observed in the tendons treated with GAGPS.
通过向10匹马左胸肢的指浅屈肌腱（SDFT）内注射细菌胶原酶诱导炎症反应。一周后，其中5匹马的肌腱（第1组）用多硫酸糖胺聚糖（GAGPS）治疗，另外5匹马的肌腱（第2组）用盐溶液治疗。胶原酶注射150天后对马实施安乐死，将SDFT样本在-14℃下冷冻，纵向和横向切成5至7μm的切片，并用天狼星红苦味酸染色法染色。形态计量分析用于量化第1组和第2组样本中有序和无序的胶原束。在用GAGPS治疗的肌腱中观察到明显更多的有序胶原束。
