Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
Analyst. 2009 Sep;134(9):1808-14. doi: 10.1039/b904476k. Epub 2009 Jul 1.
In this work, we have developed new aptamer probes for non-small cell lung cancer (NSCLC) by directing the aptamer selection process against the living cells of adenocarcinoma, the most common subtype of NSCLC. A panel of single-stranded DNA (ssDNA) aptamers were generated and evaluated for adenocarcinoma cell recognition. The aptamers bound to the adenocarcinoma cells with dissociation constants in the nanomolar range and the binding of the selected aptamers to the adenocarcinoma cells were significantly stronger than the other cancerous lung cells as well as other types of cancer cells. Moreover, the application of the aptamers to the clinical tissue section samples showed the differentiation of adenocarcinoma from normal lung tissue and other subtypes of lung cancer. The aptamers are expected to be new molecular probes for the investigation of the molecular bases of different NSCLC subtypes and their biological heterogeneity, which is valuable for advancing NSCLC diagnosis and treatment.
在这项工作中，我们通过针对非小细胞肺癌（NSCLC）中最常见的腺癌亚型的活细胞来指导适体选择过程，开发了新的适体探针。生成了一组单链 DNA（ssDNA）适体，并对其进行了评估，以确定其对腺癌细胞的识别能力。这些适体与腺癌细胞的解离常数在纳摩尔范围内，并且与所选适体与腺癌细胞的结合明显强于其他癌细胞以及其他类型的癌细胞。此外，将适体应用于临床组织切片样本中，显示了腺癌与正常肺组织和其他肺癌亚型的区分。这些适体有望成为研究不同 NSCLC 亚型及其生物学异质性的分子基础的新型分子探针，这对于推进 NSCLC 的诊断和治疗具有重要价值。
