College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Shandong Normal University, 88 East Wenhua Road, Jinan 250014, P. R. China.
Analyst. 2009 Sep;134(9):1826-33. doi: 10.1039/b823360h. Epub 2009 Jul 17.
A new fluorescent and colorimetric Cu2+ probe was synthesized, and realized optical imaging in RAW264.7 macrophages. The design strategy was based on a change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine-based dyes, and its crystal structure was presented to explain the binding mode. Upon treatment with Cu2+, the weakly fluorescent probe exhibited a strong fluorescence response, high selectivity and was quantitative for Cu2+ under physiological conditions. In addition, the off-on-type fluorescent change upon the addition of Cu2+ was also applied in bioimaging.
一种新型的荧光比色 Cu2+ 探针被合成，并在 RAW264.7 巨噬细胞中实现了光学成像。该设计策略基于螺环（非荧光）和开环（荧光）形式罗丹明类染料结构之间的变化，并提出了其晶体结构来解释结合模式。与 Cu2+ 作用后，弱荧光探针表现出强荧光响应，对 Cu2+ 具有高选择性和定量性，在生理条件下也是如此。此外，加入 Cu2+ 后呈现出的关闭-开启型荧光变化也被应用于生物成像。
