Andrioli João Luciano, Oliveira Gílvia Simone Andrade, Barreto Cilene Souza, Sousa Zulane Lima, Oliveira Maria Cristina Haun de, Cazorla Irene Mauricio, Fontana Renato
Universidade Estadual de Santa Cruz, CEP 45662-000 - Ilhéus, BA, Brasil.
Rev Bras Ginecol Obstet. 2009 Jun;31(6):300-4. doi: 10.1590/s0100-72032009000600006.
to study vulvovaginal candidiasis from the vaginal fluid of women with and without clinical suspicion, identifying the frequency of Candida spp., and associating it with intrinsic and extrinsic risk factors.
a total of 286 samples from patients attended in private practices and public health units from August 2005 to August 2007 were collected, being 121 women under clinical suspicion and 165, without. The samples were collected with sterile swabs, taken to the laboratory in 0.85% physiological solution, and then seeded in CHROMagar Candida and in 4% agar Sabourad with chloramphenicol. Classical identification procedures were carried out: macro and micromorphology, zymogram and auxanogram. Data obtained were analyzed by frequency tests and contingency tables (chi2).
a total of 47.9% of the women under clinical suspicion got confirmation of candidiasis by the laboratorial tests. Among the patients without clinical suspicion (Control Group), 78.2% were vulvovaginal candidiasis negative according to the laboratorial tests. Candida albicans was the prevalent strain in 74.5% of the cases. There were significant differences among the positive cases, according to the patients from the two cities evaluated (p<0.05). Clothing was one differential aspect found among the two populations studied.
the presence of predisposing factors does not necessarily define vulvovaginal candidiasis. Geographical localization has shown to be a relevant factor in the distribution of events. The type of clothing may be one of the reasons for it. Culture of samples from the vaginal contents, followed by microorganisms' identification, can be important.
研究有或无临床疑似症状的女性阴道分泌物中的外阴阴道念珠菌病，确定念珠菌属的感染频率，并将其与内在和外在风险因素相关联。
收集了2005年8月至2007年8月在私人诊所和公共卫生单位就诊的患者的286份样本，其中121名女性有临床疑似症状，165名没有。样本用无菌拭子采集，置于0.85%生理盐水中带到实验室，然后接种于科玛嘉念珠菌显色培养基和含氯霉素的4%沙氏琼脂培养基上。进行经典的鉴定程序：宏观和微观形态学、酶谱分析和营养谱分析。所获数据通过频率检验和列联表（卡方检验）进行分析。
在有临床疑似症状的女性中，共有47.9%通过实验室检测确诊为念珠菌病。在无临床疑似症状的患者（对照组）中，根据实验室检测，78.2%的外阴阴道念珠菌病呈阴性。白色念珠菌是74.5%病例中的优势菌株。根据所评估的两个城市的患者，阳性病例之间存在显著差异（p<0.05）。服装是在所研究的两个人群中发现的一个差异因素。
易感因素的存在不一定能确定外阴阴道念珠菌病。地理定位已被证明是事件分布中的一个相关因素。服装类型可能是其中一个原因。对阴道分泌物样本进行培养，随后进行微生物鉴定可能很重要。
