Thomas Sharon E, McKee Bruce D
Department of Biochemistry, Cellular & Molecular Biology, University of Tennessee, Knoxville, TN, USA.
Methods Mol Biol. 2009;558:217-34. doi: 10.1007/978-1-60761-103-5_13.
A wide variety of techniques have been utilized to determine the localization of various proteins from premeiotic through meiotic stages in Drosophila males. Live imaging has been instrumental in monitoring chromosome pairing and the localization of fusion proteins. Immunofluorescence has been a widely utilized technique to examine the localization and colocalization of the many proteins involved in meiosis. Recently, an immuno-FISH protocol was developed to observe the co-localization of DNA probes and proteins. In this chapter, detailed protocols outlining these three types of experiments are presented.
人们已经运用了各种各样的技术来确定果蝇雄性个体中从减数分裂前到减数分裂阶段各种蛋白质的定位。实时成像在监测染色体配对和融合蛋白的定位方面发挥了重要作用。免疫荧光是一种广泛应用的技术，用于检测参与减数分裂的许多蛋白质的定位和共定位。最近，一种免疫荧光原位杂交方案被开发出来，用于观察DNA探针和蛋白质的共定位。在本章中，将介绍概述这三种类型实验的详细方案。
