Kugou Kazuto, Ohta Kunihiro
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2009;557:285-304. doi: 10.1007/978-1-59745-527-5_18.
Cooperative actions of chromosomal proteins play critical roles in the dynamics, structural transition, segregation, and maintenance of meiotic chromosomes. A high-resolutibn genome-tiling array combined with a chromatin immunoprecipitation assay (ChIP-chip) is a powerful tool for uncovering the precise and dynamic distributions of various proteins along meiotic chromosomes. In this chapter, we describe a method to map the binding sites of meiotic chromosomal proteins such as Spo11, Mre11, and Rec8 using the high-resolution ChIP-chip technology. This system provides us with informative knowledge on the interplay of meiotic chromosomal proteins.
染色体蛋白的协同作用在减数分裂染色体的动态变化、结构转变、分离和维持过程中发挥着关键作用。高分辨率基因组平铺阵列结合染色质免疫沉淀测定法（ChIP-chip）是一种强大的工具，可用于揭示各种蛋白质沿减数分裂染色体的精确动态分布。在本章中，我们描述了一种使用高分辨率ChIP-chip技术绘制减数分裂染色体蛋白（如Spo11、Mre11和Rec8）结合位点的方法。该系统为我们提供了有关减数分裂染色体蛋白相互作用的丰富信息。
