Roig Ignasi, Garcia-Caldés Montserrat
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Methods Mol Biol. 2009;558:419-31. doi: 10.1007/978-1-60761-103-5_24.
Most of the human aneuploidies have a maternal origin. This feature makes the study of human female meiosis a fundamental topic to understand the reasons leading to this important social problem. Unfortunately, due to sample collection difficulties, not many studies have been performed on human female meiotic prophase. In this chapter we present a comprehensive collection of protocols that allows the study of human female meiotic prophase through different technical approaches using both spread and structurally preserved oocytes.
大多数人类非整倍体都源于母体。这一特征使得对人类女性减数分裂的研究成为理解导致这一重要社会问题的原因的一个基本课题。不幸的是，由于样本采集困难，针对人类女性减数分裂前期的研究并不多。在本章中，我们展示了一系列全面的实验方案，这些方案能够通过不同的技术方法，利用铺展型和结构保存型卵母细胞来研究人类女性减数分裂前期。
