固定果蝇卵巢减数分裂的细胞学分析。

Cytological analysis of meiosis in fixed Drosophila ovaries.

作者信息

McKim Kim S, Joyce Eric F, Jang Janet K

机构信息

Waksman Institute and Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.

出版信息

Methods Mol Biol. 2009;558:197-216. doi: 10.1007/978-1-60761-103-5_12.

DOI:10.1007/978-1-60761-103-5_12

PMID:19685326

Abstract

Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.

摘要

本文描述了分析果蝇卵巢两个不同部分的方法，这两个部分分别对应减数分裂的早期阶段（粗线期）和晚期阶段（减数第一次分裂中期及以后）。除了考虑形态学外，这些技术在固定条件和组织分离方法上也有所不同。这些方法大多是整装片法，可保留三维结构。