Zhu Xinhua, Liu Yuehui
Department of Otolaryngology-Head and Neck Surgery, the Second Affiliated Hospital, Nanchang University, Nanchang, 330006, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 Apr;23(8):364-8.
To observe histopathologic changes of irradiated guinea pigs' nasal mucosa treated with intranasal corticosteroids and to study the radioprotective effect of intranasal corticosteroids.
Fifty health guinea pigs nasal parts were performed irradiation by the WDVE-6MeV linear accelerator. They had accepted 5 Gy one time per week for three weeks through X-ray irradiating to establish the animal irradiation injury model. After that, they were divided into 2 groups randomly: the control group and the administration group and each group had 25 guinea pigs. The administration group received intranasal corticosteroids on the second day after three weeks irradiation, 5 animals per one group were sacrificed randomly at 1 W, 1 M, 2 M, 3 M, 4 M after administration, the histopathologic changes were observed under optical, scanning electron and transmission electron microscope respectively.
Using intranasal corticosteroids after irradiation, the early inflammatory reaction of the administration group was milder than the control group. With the drug being given constantly, the recovery of epithelial cell with irradiated damage was accelerated and the coverage rate of cilia went up obviously; After four months, the coverage rate of cilia had risen to 72.9%; But, for the control group, the coverage rate of cilia is only 50.2%. The atrophy of submucosal glandular organ was lessened and they displayed some extent secretory function. The reparation was accelerated as time went by.
Irradiation brought about serious injury on guinea pigs' nasal mucosa. But, the injury was lessen after using intranasal corticosteroids. Intranasal corticosteroids play the role of radioprotection for the irradiated nasal mucosa.
观察鼻用糖皮质激素对受照射豚鼠鼻黏膜的组织病理学变化，研究鼻用糖皮质激素的辐射防护作用。
用WDVE - 6MeV直线加速器对50只健康豚鼠鼻部进行照射。通过X线照射，每周1次，每次5 Gy，共照射3周，建立动物辐射损伤模型。之后，将其随机分为2组：对照组和给药组，每组25只豚鼠。给药组在照射3周后的第2天开始鼻用糖皮质激素，给药后1周、1个月、2个月、3个月、4个月每组随机处死5只动物，分别在光学显微镜、扫描电子显微镜和透射电子显微镜下观察组织病理学变化。
照射后使用鼻用糖皮质激素，给药组早期炎症反应较对照组轻。随着药物持续给予，受照射损伤的上皮细胞恢复加快，纤毛覆盖率明显上升；4个月后，纤毛覆盖率升至72.9%；而对照组纤毛覆盖率仅为50.2%。黏膜下腺器官萎缩减轻，显示出一定程度的分泌功能。随着时间推移，修复加快。
照射对豚鼠鼻黏膜造成严重损伤。但使用鼻用糖皮质激素后损伤减轻。鼻用糖皮质激素对受照射的鼻黏膜起到辐射防护作用。
